Ki-67 as a prognostic marker in mantle cell lymphoma-consensus guidelines of the pathology panel of the European MCL network

Wolfram Klapper, Eva Hoster, Olaf Determann, Ilske Oschlies, Jeroen van der Laak, Françoise Berger, Heinz Wolfram Bernd, José Cabeçadas, Elias Campo, Sergio Cogliatti, Martin Leo Hansmann, Philip M. Kluin, Roman Kodet, Yuri A. Krivolapov, Christoph Loddenkemper, Harald Stein, Peter Möller, Thomas E F Barth, Konrad Müller-Hermelink, Andreas RosenwaldGerman Ott, Stefano Pileri, Elisabeth Ralfkiaer, Grzegorz Rymkiewicz, Johan H. van Krieken, Hans Heinrich Wacker, Michael Unterhalt, Wolfgang Hiddemann, Martin Dreyling

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Abstract

Mantle cell lymphoma (MCL) has a heterogeneous clinical course and is mainly an aggressive B cell non-Hodgkin lymphoma; however, there are some indolent cases The Ki-67 index, defined by the percentage of Ki-67-positive lymphoma cells on histo-pathological slides, has been shown to be a very powerful prognostic biomarker. The pathology panel of the European MCL Network evaluated methods to assess the Ki-67 index including stringent counting, digital image analysis, and estimation by eyeballing. Counting of 2×500 lymphoma cells is the gold standard to assess the Ki-67 index since this value has been shown to predict survival in prospective randomized trials of the European MCL Network. Estimation by eyeballing and digital image analysis showed a poor concordance with the gold standard (concordance correlation coefficients [CCC] between 0.29 and 0.61 for eyeballing and CCC of 0.24 and 0.37 for two methods of digital image analysis, respectively). Counting a reduced number of lymphoma cells (2×100 cells) showed high interobserver agreement (CCC=0.74). Pitfalls of the Ki-67 index are discussed and guidelines and recommendations for assessing the Ki-67 index in MCL are given.

Original languageEnglish
Pages (from-to)103-111
Number of pages9
JournalJournal of Hematopathology
Volume2
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

Mantle-Cell Lymphoma
Guidelines
Pathology
Lymphoma
B-Cell Lymphoma
Non-Hodgkin's Lymphoma
Cell Count
Biomarkers

Keywords

  • Consensus guidelines
  • European MCL network
  • Ki-67
  • Mantle cell lymphoma
  • Prognostic marker

ASJC Scopus subject areas

  • Hematology
  • Pathology and Forensic Medicine
  • Histology

Cite this

Klapper, W., Hoster, E., Determann, O., Oschlies, I., van der Laak, J., Berger, F., ... Dreyling, M. (2009). Ki-67 as a prognostic marker in mantle cell lymphoma-consensus guidelines of the pathology panel of the European MCL network. Journal of Hematopathology, 2(2), 103-111. https://doi.org/10.1007/s12308-009-0036-x

Ki-67 as a prognostic marker in mantle cell lymphoma-consensus guidelines of the pathology panel of the European MCL network. / Klapper, Wolfram; Hoster, Eva; Determann, Olaf; Oschlies, Ilske; van der Laak, Jeroen; Berger, Françoise; Bernd, Heinz Wolfram; Cabeçadas, José; Campo, Elias; Cogliatti, Sergio; Leo Hansmann, Martin; Kluin, Philip M.; Kodet, Roman; Krivolapov, Yuri A.; Loddenkemper, Christoph; Stein, Harald; Möller, Peter; Barth, Thomas E F; Müller-Hermelink, Konrad; Rosenwald, Andreas; Ott, German; Pileri, Stefano; Ralfkiaer, Elisabeth; Rymkiewicz, Grzegorz; van Krieken, Johan H.; Wacker, Hans Heinrich; Unterhalt, Michael; Hiddemann, Wolfgang; Dreyling, Martin.

In: Journal of Hematopathology, Vol. 2, No. 2, 2009, p. 103-111.

Research output: Contribution to journalArticle

Klapper, W, Hoster, E, Determann, O, Oschlies, I, van der Laak, J, Berger, F, Bernd, HW, Cabeçadas, J, Campo, E, Cogliatti, S, Leo Hansmann, M, Kluin, PM, Kodet, R, Krivolapov, YA, Loddenkemper, C, Stein, H, Möller, P, Barth, TEF, Müller-Hermelink, K, Rosenwald, A, Ott, G, Pileri, S, Ralfkiaer, E, Rymkiewicz, G, van Krieken, JH, Wacker, HH, Unterhalt, M, Hiddemann, W & Dreyling, M 2009, 'Ki-67 as a prognostic marker in mantle cell lymphoma-consensus guidelines of the pathology panel of the European MCL network', Journal of Hematopathology, vol. 2, no. 2, pp. 103-111. https://doi.org/10.1007/s12308-009-0036-x
Klapper, Wolfram ; Hoster, Eva ; Determann, Olaf ; Oschlies, Ilske ; van der Laak, Jeroen ; Berger, Françoise ; Bernd, Heinz Wolfram ; Cabeçadas, José ; Campo, Elias ; Cogliatti, Sergio ; Leo Hansmann, Martin ; Kluin, Philip M. ; Kodet, Roman ; Krivolapov, Yuri A. ; Loddenkemper, Christoph ; Stein, Harald ; Möller, Peter ; Barth, Thomas E F ; Müller-Hermelink, Konrad ; Rosenwald, Andreas ; Ott, German ; Pileri, Stefano ; Ralfkiaer, Elisabeth ; Rymkiewicz, Grzegorz ; van Krieken, Johan H. ; Wacker, Hans Heinrich ; Unterhalt, Michael ; Hiddemann, Wolfgang ; Dreyling, Martin. / Ki-67 as a prognostic marker in mantle cell lymphoma-consensus guidelines of the pathology panel of the European MCL network. In: Journal of Hematopathology. 2009 ; Vol. 2, No. 2. pp. 103-111.
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T1 - Ki-67 as a prognostic marker in mantle cell lymphoma-consensus guidelines of the pathology panel of the European MCL network

AU - Klapper, Wolfram

AU - Hoster, Eva

AU - Determann, Olaf

AU - Oschlies, Ilske

AU - van der Laak, Jeroen

AU - Berger, Françoise

AU - Bernd, Heinz Wolfram

AU - Cabeçadas, José

AU - Campo, Elias

AU - Cogliatti, Sergio

AU - Leo Hansmann, Martin

AU - Kluin, Philip M.

AU - Kodet, Roman

AU - Krivolapov, Yuri A.

AU - Loddenkemper, Christoph

AU - Stein, Harald

AU - Möller, Peter

AU - Barth, Thomas E F

AU - Müller-Hermelink, Konrad

AU - Rosenwald, Andreas

AU - Ott, German

AU - Pileri, Stefano

AU - Ralfkiaer, Elisabeth

AU - Rymkiewicz, Grzegorz

AU - van Krieken, Johan H.

AU - Wacker, Hans Heinrich

AU - Unterhalt, Michael

AU - Hiddemann, Wolfgang

AU - Dreyling, Martin

PY - 2009

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N2 - Mantle cell lymphoma (MCL) has a heterogeneous clinical course and is mainly an aggressive B cell non-Hodgkin lymphoma; however, there are some indolent cases The Ki-67 index, defined by the percentage of Ki-67-positive lymphoma cells on histo-pathological slides, has been shown to be a very powerful prognostic biomarker. The pathology panel of the European MCL Network evaluated methods to assess the Ki-67 index including stringent counting, digital image analysis, and estimation by eyeballing. Counting of 2×500 lymphoma cells is the gold standard to assess the Ki-67 index since this value has been shown to predict survival in prospective randomized trials of the European MCL Network. Estimation by eyeballing and digital image analysis showed a poor concordance with the gold standard (concordance correlation coefficients [CCC] between 0.29 and 0.61 for eyeballing and CCC of 0.24 and 0.37 for two methods of digital image analysis, respectively). Counting a reduced number of lymphoma cells (2×100 cells) showed high interobserver agreement (CCC=0.74). Pitfalls of the Ki-67 index are discussed and guidelines and recommendations for assessing the Ki-67 index in MCL are given.

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