Ki-67 grading of nonfunctioning pancreatic neuroendocrine tumors on histologic samples obtained by EUS-guided fine-needle tissue acquisition: A prospective study

Alberto Larghi, Gabriele Capurso, Antonella Carnuccio, Riccardo Ricci, Sergio Alfieri, Domenico Galasso, Francesca Lugli, Antonio Bianchi, Francesco Panzuto, Laura De Marinis, Massimo Falconi, Gianfranco Delle Fave, Giovanni Battista Doglietto, Guido Costamagna, Guido Rindi

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Preoperative determination of Ki-67 expression, an important prognostic factor for grading nonfunctioning pancreatic endocrine tumors (NF-PETs), remains an important clinical challenge. To prospectively evaluate the feasibility, yield, and clinical impact of EUS-guided fine-needle tissue acquisition (EUS-FNTA) with a large-gauge needle to obtain tissue samples for histologic diagnosis and Ki-67 analysis in patients with suspected NF-PETs. Prospective cohort study. Tertiary-care academic medical center. Consecutive patients with a single pancreatic lesion suspicious for NF-PET on imaging. EUS-FNTA with a 19-gauge needle. Feasibility and yield of EUS-FNTA for diagnosis and Ki-67 expression determination. Thirty patients (mean [± SD] age 55.7 ± 14.9 years), with a mean (± SD) lesion size of 16.9 ± 6.1 mm were enrolled. EUS-FNTA was successfully performed without complications in all patients, with a mean (± SD) of 2.7 ± 0.5 passes per patient. Adequate samples for histologic examination were obtained in 28 of the 30 patients (93.3%). Ki-67 determination could be performed in 26 of these 28 patients (92.9%, 86.6% overall), 12 of whom underwent surgical resection. Preoperative and postoperative Ki-67 proliferation indexes were concordant in 10 patients (83.3%), whereas 2 patients were upstaged from G1 to G2 or downstaged from G2 to G1, respectively. Single center study with a single operator. In patients with suspected nonfunctioning low-grade to intermediate-grade pancreatic neuroendocrine tumors (p-NETs), retrieval of tissue specimens with EUS-FNTA by using a 19-gauge needle is safe, feasible, and highly accurate for both diagnosis and Ki-67 determination. A Ki-67 proliferative index acquired through this technique might be of great help for further therapeutic decisions.

Original languageEnglish
Pages (from-to)570-577
Number of pages8
JournalGastrointestinal Endoscopy
Volume76
Issue number3
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Neuroendocrine Tumors
Needles
Prospective Studies
Neoplasms
Tertiary Healthcare
Cohort Studies

ASJC Scopus subject areas

  • Gastroenterology
  • Radiology Nuclear Medicine and imaging

Cite this

Ki-67 grading of nonfunctioning pancreatic neuroendocrine tumors on histologic samples obtained by EUS-guided fine-needle tissue acquisition : A prospective study. / Larghi, Alberto; Capurso, Gabriele; Carnuccio, Antonella; Ricci, Riccardo; Alfieri, Sergio; Galasso, Domenico; Lugli, Francesca; Bianchi, Antonio; Panzuto, Francesco; De Marinis, Laura; Falconi, Massimo; Delle Fave, Gianfranco; Doglietto, Giovanni Battista; Costamagna, Guido; Rindi, Guido.

In: Gastrointestinal Endoscopy, Vol. 76, No. 3, 09.2012, p. 570-577.

Research output: Contribution to journalArticle

Larghi, A, Capurso, G, Carnuccio, A, Ricci, R, Alfieri, S, Galasso, D, Lugli, F, Bianchi, A, Panzuto, F, De Marinis, L, Falconi, M, Delle Fave, G, Doglietto, GB, Costamagna, G & Rindi, G 2012, 'Ki-67 grading of nonfunctioning pancreatic neuroendocrine tumors on histologic samples obtained by EUS-guided fine-needle tissue acquisition: A prospective study', Gastrointestinal Endoscopy, vol. 76, no. 3, pp. 570-577. https://doi.org/10.1016/j.gie.2012.04.477
Larghi, Alberto ; Capurso, Gabriele ; Carnuccio, Antonella ; Ricci, Riccardo ; Alfieri, Sergio ; Galasso, Domenico ; Lugli, Francesca ; Bianchi, Antonio ; Panzuto, Francesco ; De Marinis, Laura ; Falconi, Massimo ; Delle Fave, Gianfranco ; Doglietto, Giovanni Battista ; Costamagna, Guido ; Rindi, Guido. / Ki-67 grading of nonfunctioning pancreatic neuroendocrine tumors on histologic samples obtained by EUS-guided fine-needle tissue acquisition : A prospective study. In: Gastrointestinal Endoscopy. 2012 ; Vol. 76, No. 3. pp. 570-577.
@article{ef505c238ae24415bf24bc9a9f5084d0,
title = "Ki-67 grading of nonfunctioning pancreatic neuroendocrine tumors on histologic samples obtained by EUS-guided fine-needle tissue acquisition: A prospective study",
abstract = "Preoperative determination of Ki-67 expression, an important prognostic factor for grading nonfunctioning pancreatic endocrine tumors (NF-PETs), remains an important clinical challenge. To prospectively evaluate the feasibility, yield, and clinical impact of EUS-guided fine-needle tissue acquisition (EUS-FNTA) with a large-gauge needle to obtain tissue samples for histologic diagnosis and Ki-67 analysis in patients with suspected NF-PETs. Prospective cohort study. Tertiary-care academic medical center. Consecutive patients with a single pancreatic lesion suspicious for NF-PET on imaging. EUS-FNTA with a 19-gauge needle. Feasibility and yield of EUS-FNTA for diagnosis and Ki-67 expression determination. Thirty patients (mean [± SD] age 55.7 ± 14.9 years), with a mean (± SD) lesion size of 16.9 ± 6.1 mm were enrolled. EUS-FNTA was successfully performed without complications in all patients, with a mean (± SD) of 2.7 ± 0.5 passes per patient. Adequate samples for histologic examination were obtained in 28 of the 30 patients (93.3{\%}). Ki-67 determination could be performed in 26 of these 28 patients (92.9{\%}, 86.6{\%} overall), 12 of whom underwent surgical resection. Preoperative and postoperative Ki-67 proliferation indexes were concordant in 10 patients (83.3{\%}), whereas 2 patients were upstaged from G1 to G2 or downstaged from G2 to G1, respectively. Single center study with a single operator. In patients with suspected nonfunctioning low-grade to intermediate-grade pancreatic neuroendocrine tumors (p-NETs), retrieval of tissue specimens with EUS-FNTA by using a 19-gauge needle is safe, feasible, and highly accurate for both diagnosis and Ki-67 determination. A Ki-67 proliferative index acquired through this technique might be of great help for further therapeutic decisions.",
author = "Alberto Larghi and Gabriele Capurso and Antonella Carnuccio and Riccardo Ricci and Sergio Alfieri and Domenico Galasso and Francesca Lugli and Antonio Bianchi and Francesco Panzuto and {De Marinis}, Laura and Massimo Falconi and {Delle Fave}, Gianfranco and Doglietto, {Giovanni Battista} and Guido Costamagna and Guido Rindi",
year = "2012",
month = "9",
doi = "10.1016/j.gie.2012.04.477",
language = "English",
volume = "76",
pages = "570--577",
journal = "Gastrointestinal Endoscopy",
issn = "0016-5107",
publisher = "Mosby Inc.",
number = "3",

}

TY - JOUR

T1 - Ki-67 grading of nonfunctioning pancreatic neuroendocrine tumors on histologic samples obtained by EUS-guided fine-needle tissue acquisition

T2 - A prospective study

AU - Larghi, Alberto

AU - Capurso, Gabriele

AU - Carnuccio, Antonella

AU - Ricci, Riccardo

AU - Alfieri, Sergio

AU - Galasso, Domenico

AU - Lugli, Francesca

AU - Bianchi, Antonio

AU - Panzuto, Francesco

AU - De Marinis, Laura

AU - Falconi, Massimo

AU - Delle Fave, Gianfranco

AU - Doglietto, Giovanni Battista

AU - Costamagna, Guido

AU - Rindi, Guido

PY - 2012/9

Y1 - 2012/9

N2 - Preoperative determination of Ki-67 expression, an important prognostic factor for grading nonfunctioning pancreatic endocrine tumors (NF-PETs), remains an important clinical challenge. To prospectively evaluate the feasibility, yield, and clinical impact of EUS-guided fine-needle tissue acquisition (EUS-FNTA) with a large-gauge needle to obtain tissue samples for histologic diagnosis and Ki-67 analysis in patients with suspected NF-PETs. Prospective cohort study. Tertiary-care academic medical center. Consecutive patients with a single pancreatic lesion suspicious for NF-PET on imaging. EUS-FNTA with a 19-gauge needle. Feasibility and yield of EUS-FNTA for diagnosis and Ki-67 expression determination. Thirty patients (mean [± SD] age 55.7 ± 14.9 years), with a mean (± SD) lesion size of 16.9 ± 6.1 mm were enrolled. EUS-FNTA was successfully performed without complications in all patients, with a mean (± SD) of 2.7 ± 0.5 passes per patient. Adequate samples for histologic examination were obtained in 28 of the 30 patients (93.3%). Ki-67 determination could be performed in 26 of these 28 patients (92.9%, 86.6% overall), 12 of whom underwent surgical resection. Preoperative and postoperative Ki-67 proliferation indexes were concordant in 10 patients (83.3%), whereas 2 patients were upstaged from G1 to G2 or downstaged from G2 to G1, respectively. Single center study with a single operator. In patients with suspected nonfunctioning low-grade to intermediate-grade pancreatic neuroendocrine tumors (p-NETs), retrieval of tissue specimens with EUS-FNTA by using a 19-gauge needle is safe, feasible, and highly accurate for both diagnosis and Ki-67 determination. A Ki-67 proliferative index acquired through this technique might be of great help for further therapeutic decisions.

AB - Preoperative determination of Ki-67 expression, an important prognostic factor for grading nonfunctioning pancreatic endocrine tumors (NF-PETs), remains an important clinical challenge. To prospectively evaluate the feasibility, yield, and clinical impact of EUS-guided fine-needle tissue acquisition (EUS-FNTA) with a large-gauge needle to obtain tissue samples for histologic diagnosis and Ki-67 analysis in patients with suspected NF-PETs. Prospective cohort study. Tertiary-care academic medical center. Consecutive patients with a single pancreatic lesion suspicious for NF-PET on imaging. EUS-FNTA with a 19-gauge needle. Feasibility and yield of EUS-FNTA for diagnosis and Ki-67 expression determination. Thirty patients (mean [± SD] age 55.7 ± 14.9 years), with a mean (± SD) lesion size of 16.9 ± 6.1 mm were enrolled. EUS-FNTA was successfully performed without complications in all patients, with a mean (± SD) of 2.7 ± 0.5 passes per patient. Adequate samples for histologic examination were obtained in 28 of the 30 patients (93.3%). Ki-67 determination could be performed in 26 of these 28 patients (92.9%, 86.6% overall), 12 of whom underwent surgical resection. Preoperative and postoperative Ki-67 proliferation indexes were concordant in 10 patients (83.3%), whereas 2 patients were upstaged from G1 to G2 or downstaged from G2 to G1, respectively. Single center study with a single operator. In patients with suspected nonfunctioning low-grade to intermediate-grade pancreatic neuroendocrine tumors (p-NETs), retrieval of tissue specimens with EUS-FNTA by using a 19-gauge needle is safe, feasible, and highly accurate for both diagnosis and Ki-67 determination. A Ki-67 proliferative index acquired through this technique might be of great help for further therapeutic decisions.

UR - http://www.scopus.com/inward/record.url?scp=84865126385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865126385&partnerID=8YFLogxK

U2 - 10.1016/j.gie.2012.04.477

DO - 10.1016/j.gie.2012.04.477

M3 - Article

C2 - 22898415

AN - SCOPUS:84865126385

VL - 76

SP - 570

EP - 577

JO - Gastrointestinal Endoscopy

JF - Gastrointestinal Endoscopy

SN - 0016-5107

IS - 3

ER -