TY - JOUR
T1 - KIBRA gene methylation is associated with unfavorable biological prognostic parameters in chronic lymphocytic leukemia
AU - Shinawi, Thoraia
AU - Hill, Victoria
AU - Dagklis, Antonis
AU - Baliakas, Panagiotis
AU - Stamatopoulos, Kostas
AU - Agathanggelou, Angleo
AU - Stankovic, Tanja
AU - Maher, Eamonn R.
AU - Ghia, Paolo
AU - Latif, Farida
PY - 2012/3
Y1 - 2012/3
N2 - Ras-association domain family (RASSF) members are a family of genes containing an RA domain in either the C-terminus (RASSF1-RASSF6) or in the N-terminus (RASSF7-RASSF10). Members of this gene family are core members of the Salvador/ Warts/Hippo (SWH) tumor suppressor network and have been shown to be involved in human tumorigenesis. Among the RASSF genes, RASSF1A is one of the most frequently methylated genes in a wide range of epithelial cancers, and we previously demonstrated that RASSF6 and RASSF10 genes are frequently epigenetically inactivated in acute leukemias, particularly in those of the B cell type. We here determined the methylation profiles of all members of the RASSF gene family as well as two recently identified (KIBRA, CRB3) upstream members of the SWH pathway in the leukemic B cells obtained from a well-characterized cohort of 95 patients with chronic lymphocytic leukemia (CLL). Among the RASSF genes, RASSF10 (50%) was the most frequently methylated gene, followed by RASSF6 (16%). The remaining RASSF genes were either unmethylated or showed a frequency of methylation
AB - Ras-association domain family (RASSF) members are a family of genes containing an RA domain in either the C-terminus (RASSF1-RASSF6) or in the N-terminus (RASSF7-RASSF10). Members of this gene family are core members of the Salvador/ Warts/Hippo (SWH) tumor suppressor network and have been shown to be involved in human tumorigenesis. Among the RASSF genes, RASSF1A is one of the most frequently methylated genes in a wide range of epithelial cancers, and we previously demonstrated that RASSF6 and RASSF10 genes are frequently epigenetically inactivated in acute leukemias, particularly in those of the B cell type. We here determined the methylation profiles of all members of the RASSF gene family as well as two recently identified (KIBRA, CRB3) upstream members of the SWH pathway in the leukemic B cells obtained from a well-characterized cohort of 95 patients with chronic lymphocytic leukemia (CLL). Among the RASSF genes, RASSF10 (50%) was the most frequently methylated gene, followed by RASSF6 (16%). The remaining RASSF genes were either unmethylated or showed a frequency of methylation
KW - Chronic lymphocytic leukemia
KW - DNA methylation
KW - KIBRA
KW - Prognostic marker
KW - RASSF genes
KW - SWH pathway
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U2 - 10.4161/epi.7.3.19222
DO - 10.4161/epi.7.3.19222
M3 - Article
C2 - 22430796
AN - SCOPUS:84858220295
VL - 7
SP - 211
EP - 215
JO - Epigenetics
JF - Epigenetics
SN - 1559-2294
IS - 3
ER -