Kidney-derived mesenchymal stromal cells modulate dendritic cell function to suppress alloimmune responses and delay allograft rejection

Yanfei Huang, Ping Chen, Cassie B. Zhang, Gang Jee Ko, Miriam Ruiz, Paolo Fiorina, Mehboob A. Hussain, Barbara A. Wasowska, Hamid Rabb, Karl L. Womer

Research output: Contribution to journalArticlepeer-review

Abstract

Background. Mesenchymal stromal cells (MSCs) are multipotent cells with immunoregulatory capacity that are present in most adult organs. We previously demonstrated that co-culture of C57BL/6 kidney-derived MSCs (KSCs) in syngeneic bone marrow-derived dendritic cell (DC) culture induced a DC phenotype (KSC-DC) with reduced major histocompatibility complex (MHC) class II/increased CD80 expression and ability to suppress T-cell responses. Methods. To study their effects on allogeneic DCs, C57BL/6 KSCs were added to incipient BALB/c DC culture, with surface expression of MHC class II/CD80 measured by fluorescence-activated cell sorting. The ability to stimulate T-cell responses was then assessed in an allogeneic mixed leukocyte response. Next, we isolated either BALB/c (donor) or C57BL/6 (recipient) KSC-DCs from co-culture and measured the tempo of rejection after cotransplantation with islet grafts in a mouse model of islet transplantation. Finally, we measured the effects of KSC-DC stimulation on B-cell proliferation and IgM/IgG production in allogeneic cultures. Results. C57BL/6 KSCs induced a BALB/c DC phenotype with significantly decreased MHC class II, increased CD80 expression, and decreased T-cell stimulatory capacity in the mixed leukocyte response (P

Original languageEnglish
Pages (from-to)1307-1311
Number of pages5
JournalTransplantation
Volume90
Issue number12
DOIs
Publication statusPublished - Dec 27 2010

Keywords

  • Antigen-presenting cells
  • Dendritic cells
  • Islet transplantation
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Transplantation

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