Abstract
Background. Mesenchymal stromal cells (MSCs) are multipotent cells with immunoregulatory capacity that are present in most adult organs. We previously demonstrated that co-culture of C57BL/6 kidney-derived MSCs (KSCs) in syngeneic bone marrow-derived dendritic cell (DC) culture induced a DC phenotype (KSC-DC) with reduced major histocompatibility complex (MHC) class II/increased CD80 expression and ability to suppress T-cell responses. Methods. To study their effects on allogeneic DCs, C57BL/6 KSCs were added to incipient BALB/c DC culture, with surface expression of MHC class II/CD80 measured by fluorescence-activated cell sorting. The ability to stimulate T-cell responses was then assessed in an allogeneic mixed leukocyte response. Next, we isolated either BALB/c (donor) or C57BL/6 (recipient) KSC-DCs from co-culture and measured the tempo of rejection after cotransplantation with islet grafts in a mouse model of islet transplantation. Finally, we measured the effects of KSC-DC stimulation on B-cell proliferation and IgM/IgG production in allogeneic cultures. Results. C57BL/6 KSCs induced a BALB/c DC phenotype with significantly decreased MHC class II, increased CD80 expression, and decreased T-cell stimulatory capacity in the mixed leukocyte response (P
| Original language | English |
|---|---|
| Pages (from-to) | 1307-1311 |
| Number of pages | 5 |
| Journal | Transplantation |
| Volume | 90 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 27 2010 |
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Keywords
- Antigen-presenting cells
- Dendritic cells
- Islet transplantation
- Mesenchymal stem cells
ASJC Scopus subject areas
- Transplantation
Cite this
Kidney-derived mesenchymal stromal cells modulate dendritic cell function to suppress alloimmune responses and delay allograft rejection. / Huang, Yanfei; Chen, Ping; Zhang, Cassie B.; Ko, Gang Jee; Ruiz, Miriam; Fiorina, Paolo; Hussain, Mehboob A.; Wasowska, Barbara A.; Rabb, Hamid; Womer, Karl L.
In: Transplantation, Vol. 90, No. 12, 27.12.2010, p. 1307-1311.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Kidney-derived mesenchymal stromal cells modulate dendritic cell function to suppress alloimmune responses and delay allograft rejection
AU - Huang, Yanfei
AU - Chen, Ping
AU - Zhang, Cassie B.
AU - Ko, Gang Jee
AU - Ruiz, Miriam
AU - Fiorina, Paolo
AU - Hussain, Mehboob A.
AU - Wasowska, Barbara A.
AU - Rabb, Hamid
AU - Womer, Karl L.
PY - 2010/12/27
Y1 - 2010/12/27
N2 - Background. Mesenchymal stromal cells (MSCs) are multipotent cells with immunoregulatory capacity that are present in most adult organs. We previously demonstrated that co-culture of C57BL/6 kidney-derived MSCs (KSCs) in syngeneic bone marrow-derived dendritic cell (DC) culture induced a DC phenotype (KSC-DC) with reduced major histocompatibility complex (MHC) class II/increased CD80 expression and ability to suppress T-cell responses. Methods. To study their effects on allogeneic DCs, C57BL/6 KSCs were added to incipient BALB/c DC culture, with surface expression of MHC class II/CD80 measured by fluorescence-activated cell sorting. The ability to stimulate T-cell responses was then assessed in an allogeneic mixed leukocyte response. Next, we isolated either BALB/c (donor) or C57BL/6 (recipient) KSC-DCs from co-culture and measured the tempo of rejection after cotransplantation with islet grafts in a mouse model of islet transplantation. Finally, we measured the effects of KSC-DC stimulation on B-cell proliferation and IgM/IgG production in allogeneic cultures. Results. C57BL/6 KSCs induced a BALB/c DC phenotype with significantly decreased MHC class II, increased CD80 expression, and decreased T-cell stimulatory capacity in the mixed leukocyte response (P
AB - Background. Mesenchymal stromal cells (MSCs) are multipotent cells with immunoregulatory capacity that are present in most adult organs. We previously demonstrated that co-culture of C57BL/6 kidney-derived MSCs (KSCs) in syngeneic bone marrow-derived dendritic cell (DC) culture induced a DC phenotype (KSC-DC) with reduced major histocompatibility complex (MHC) class II/increased CD80 expression and ability to suppress T-cell responses. Methods. To study their effects on allogeneic DCs, C57BL/6 KSCs were added to incipient BALB/c DC culture, with surface expression of MHC class II/CD80 measured by fluorescence-activated cell sorting. The ability to stimulate T-cell responses was then assessed in an allogeneic mixed leukocyte response. Next, we isolated either BALB/c (donor) or C57BL/6 (recipient) KSC-DCs from co-culture and measured the tempo of rejection after cotransplantation with islet grafts in a mouse model of islet transplantation. Finally, we measured the effects of KSC-DC stimulation on B-cell proliferation and IgM/IgG production in allogeneic cultures. Results. C57BL/6 KSCs induced a BALB/c DC phenotype with significantly decreased MHC class II, increased CD80 expression, and decreased T-cell stimulatory capacity in the mixed leukocyte response (P
KW - Antigen-presenting cells
KW - Dendritic cells
KW - Islet transplantation
KW - Mesenchymal stem cells
UR - http://www.scopus.com/inward/record.url?scp=78650842941&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650842941&partnerID=8YFLogxK
U2 - 10.1097/TP.0b013e3181fdd9eb
DO - 10.1097/TP.0b013e3181fdd9eb
M3 - Article
VL - 90
SP - 1307
EP - 1311
JO - Transplantation
JF - Transplantation
SN - 0041-1337
IS - 12
ER -