KIF3A and IL-4 are disease-specific biomarkers for psoriatic arthritis susceptibility

Raffaella Cascella, Claudia Strafella, Michele Ragazzo, Laura Manzo, Gaetana Costanza, John Bowes, Ulrike Hüffmeier, Saverio Potenza, Federica Sangiuolo, André Reis, Anne Barton, Giuseppe Novelli, Augusto Orlandi, Emiliano Giardina

Research output: Contribution to journalArticle

Abstract

To date, the genes associated with Psoriatic Arthritis (PsA) are principally involved in inflammation, immune response and epidermal differentiation, without any information about the relationship between disease and bone metabolism genes. Our work was focused on 5q31 locus, which contains several genetic variants significantly associated with PsA. The study involved 1526 subjects (500 PsA, 426 PsV, 600 controls). The region was evaluated by selecting and genotyping the SNPs of interest by Real Time PCR and direct sequencing. The results were subjected to biostatistic and bioinformatic analysis. The case-control study highlighted a significant association between KIF3A/IL- 4 and PsA, but not with PsV (Psoriasis Vulgaris) patients. In addition, the haplotype analysis revealed two haplotypes significantly associated with PsA susceptibility. The Linkage Disequilibrium (LD) study showed the presence of a specific block in high LD within 132,692,628-132,737,638 bp of 5q31, giving additional evidence of specific association of the 5q31 region in PsA patients. Moreover, KIF3A expression was assessed by immunohistochemistry assays which showed a marked and significant difference of KIF3A expression between pathological and normal tissues. Our analysis described KIF3A and IL-4 as novel susceptibility genes for PsA, suggesting a clear implication of bone metabolism genes in the disease etiopathogenesis.

Original languageEnglish
Pages (from-to)95401-95411
Number of pages11
JournalOncotarget
Volume8
Issue number56
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • 5q31 locus
  • Bone metabolism
  • Linkage disequilibrium
  • Psoriatic arthritis
  • Susceptibility

ASJC Scopus subject areas

  • Oncology

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    Cascella, R., Strafella, C., Ragazzo, M., Manzo, L., Costanza, G., Bowes, J., Hüffmeier, U., Potenza, S., Sangiuolo, F., Reis, A., Barton, A., Novelli, G., Orlandi, A., & Giardina, E. (2017). KIF3A and IL-4 are disease-specific biomarkers for psoriatic arthritis susceptibility. Oncotarget, 8(56), 95401-95411. https://doi.org/10.18632/oncotarget.20727