Abstract
Endothelial progenitor cells (EPCs) have been suggested to contribute to the neovascularization of infantile haemangioma (IH). There is strong evidence of the efficacy of propranolol in the treatment of IH, possibly by inhibiting both vasculogenesis and angiogenesis in the tumour. We evaluate the frequency of circulating endothelial colony forming cells (ECFCs), as the best EPC surrogate, in patients with IH at diagnosis and while receiving propranolol by an ex vivo 12-month longitudinal study. Biological aspects of the ECFCs, such as their in vitro angiogenic potential, membrane CXCR4 expression and Ca 2+ signalling, were investigated. Circulating ECFCs were isolated by in vitro culture and expanded for 2 to 3 passages in 23 patients with IH (median age: 5.5 months, range: 5.5 weeks-11 months) before and 3, 6, 9 and 12 months after receiving propranolol. Twenty-four healthy subjects comparable for age were also assessed (CTRLs). Untreated patients with IH had a circulating ECFC frequency lower (p = 0.001) than CTRLs; nevertheless, in in vitro starving conditions, ECFCs showed enhanced capacity to form tube-like structures than those of CTRLs. Patients with IH following the therapy with propranolol had a significantly increased (p = 0.022) circulating ECFC frequency, that showed a diminished tube-like formation capacity in vitro, and an altered constitutive store-operated Ca 2+ entry. ECFCs play a role in IH pathogenesis; the response to propranolol therapy is associated with their increased frequency in the peripheral blood and a reduction of their vasculogenic activity.
| Original language | English |
|---|---|
| Article number | 180507 |
| Pages (from-to) | 274-284 |
| Number of pages | 11 |
| Journal | Thrombosis and Haemostasis |
| Volume | 119 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 2019 |
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Keywords
- angiogenesis
- endothelial colony forming cells
- endothelial progenitor cells
- infantile haemangioma
- propranolol
ASJC Scopus subject areas
- Hematology
Cite this
Kinetic and Angiogenic Activity of Circulating Endothelial Colony Forming Cells in Patients with Infantile Haemangioma Receiving Propranolol. / Campanelli, Rita; Codazzi, Alessia Claudia; Poletto, Valentina; Abbà, Carlotta; Catarsi, Paolo; Fois, Gabriela; Avanzini, Maria Antonietta; Brazzelli, Valeria; Tzialla, Chryssoula; De Silvestri, Annalisa; Tinelli, Carmine; Licari, Amelia; Berra-Romani, Roberto; Zuccolo, Estella; Moccia, Francesco; Mannarino, Savina; Rosti, Vittorio; Massa, Margherita.
In: Thrombosis and Haemostasis, Vol. 119, No. 2, 180507, 01.01.2019, p. 274-284.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Kinetic and Angiogenic Activity of Circulating Endothelial Colony Forming Cells in Patients with Infantile Haemangioma Receiving Propranolol
AU - Campanelli, Rita
AU - Codazzi, Alessia Claudia
AU - Poletto, Valentina
AU - Abbà, Carlotta
AU - Catarsi, Paolo
AU - Fois, Gabriela
AU - Avanzini, Maria Antonietta
AU - Brazzelli, Valeria
AU - Tzialla, Chryssoula
AU - De Silvestri, Annalisa
AU - Tinelli, Carmine
AU - Licari, Amelia
AU - Berra-Romani, Roberto
AU - Zuccolo, Estella
AU - Moccia, Francesco
AU - Mannarino, Savina
AU - Rosti, Vittorio
AU - Massa, Margherita
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Endothelial progenitor cells (EPCs) have been suggested to contribute to the neovascularization of infantile haemangioma (IH). There is strong evidence of the efficacy of propranolol in the treatment of IH, possibly by inhibiting both vasculogenesis and angiogenesis in the tumour. We evaluate the frequency of circulating endothelial colony forming cells (ECFCs), as the best EPC surrogate, in patients with IH at diagnosis and while receiving propranolol by an ex vivo 12-month longitudinal study. Biological aspects of the ECFCs, such as their in vitro angiogenic potential, membrane CXCR4 expression and Ca 2+ signalling, were investigated. Circulating ECFCs were isolated by in vitro culture and expanded for 2 to 3 passages in 23 patients with IH (median age: 5.5 months, range: 5.5 weeks-11 months) before and 3, 6, 9 and 12 months after receiving propranolol. Twenty-four healthy subjects comparable for age were also assessed (CTRLs). Untreated patients with IH had a circulating ECFC frequency lower (p = 0.001) than CTRLs; nevertheless, in in vitro starving conditions, ECFCs showed enhanced capacity to form tube-like structures than those of CTRLs. Patients with IH following the therapy with propranolol had a significantly increased (p = 0.022) circulating ECFC frequency, that showed a diminished tube-like formation capacity in vitro, and an altered constitutive store-operated Ca 2+ entry. ECFCs play a role in IH pathogenesis; the response to propranolol therapy is associated with their increased frequency in the peripheral blood and a reduction of their vasculogenic activity.
AB - Endothelial progenitor cells (EPCs) have been suggested to contribute to the neovascularization of infantile haemangioma (IH). There is strong evidence of the efficacy of propranolol in the treatment of IH, possibly by inhibiting both vasculogenesis and angiogenesis in the tumour. We evaluate the frequency of circulating endothelial colony forming cells (ECFCs), as the best EPC surrogate, in patients with IH at diagnosis and while receiving propranolol by an ex vivo 12-month longitudinal study. Biological aspects of the ECFCs, such as their in vitro angiogenic potential, membrane CXCR4 expression and Ca 2+ signalling, were investigated. Circulating ECFCs were isolated by in vitro culture and expanded for 2 to 3 passages in 23 patients with IH (median age: 5.5 months, range: 5.5 weeks-11 months) before and 3, 6, 9 and 12 months after receiving propranolol. Twenty-four healthy subjects comparable for age were also assessed (CTRLs). Untreated patients with IH had a circulating ECFC frequency lower (p = 0.001) than CTRLs; nevertheless, in in vitro starving conditions, ECFCs showed enhanced capacity to form tube-like structures than those of CTRLs. Patients with IH following the therapy with propranolol had a significantly increased (p = 0.022) circulating ECFC frequency, that showed a diminished tube-like formation capacity in vitro, and an altered constitutive store-operated Ca 2+ entry. ECFCs play a role in IH pathogenesis; the response to propranolol therapy is associated with their increased frequency in the peripheral blood and a reduction of their vasculogenic activity.
KW - angiogenesis
KW - endothelial colony forming cells
KW - endothelial progenitor cells
KW - infantile haemangioma
KW - propranolol
UR - http://www.scopus.com/inward/record.url?scp=85060379322&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060379322&partnerID=8YFLogxK
U2 - 10.1055/s-0038-1676855
DO - 10.1055/s-0038-1676855
M3 - Article
C2 - 30609443
AN - SCOPUS:85060379322
VL - 119
SP - 274
EP - 284
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
SN - 0340-6245
IS - 2
M1 - 180507
ER -