Kinetics and intracellular compartmentalization of HTLV-1 gene expression

Nuclear retention of HBZ mRNAs

Francesca Rende, Ilaria Cavallari, Alberto Corradin, Micol Silic-Benussi, Frederic Toulza, Gianna M. Toffolo, Yuetsu Tanaka, Steven Jacobson, Graham P. Taylor, Donna M. D'Agostino, Charles R M Bangham, Vincenzo Ciminale

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Human T-cell leukemia virus type 1 (HTLV-1) codes for 9 alternatively spliced transcripts and 2 major regulatory proteins named Tax and Rex that function at the transcriptional and posttranscriptional levels, respectively. We investigated the temporal sequence of HTLV-1 gene expression in primary cells from infected patients using splice site-specific quantitative RT-PCR. The results indicated a two-phase kinetics with the tax/rex mRNA preceding expression of other viral transcripts. Analysis of mRNA compartmentalization in cells transfected with HTLV-1 molecular clones demonstrated the strict Rex-dependency of the two-phase kinetics and revealed strong nuclear retention of HBZ mRNAs, supporting their function as noncoding transcripts. Mathematical modeling underscored the importance of a delay between the functions of Tax and Rex, which was supported by experimental evidence of the longer half-life of Rex. These data provide evidence for a temporal pattern of HTLV-1 expression and reveal major differences in the intracellular compartmentalization of HTLV-1 transcripts.

Original languageEnglish
Pages (from-to)4855-4859
Number of pages5
JournalBlood
Volume117
Issue number18
DOIs
Publication statusPublished - May 5 2011

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Deltaretrovirus
T-cells
Viruses
Gene expression
Gene Expression
Messenger RNA
Taxation
Kinetics
rex Gene Products
tax Gene Products
Half-Life
Clone Cells
Polymerase Chain Reaction
Proteins

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Rende, F., Cavallari, I., Corradin, A., Silic-Benussi, M., Toulza, F., Toffolo, G. M., ... Ciminale, V. (2011). Kinetics and intracellular compartmentalization of HTLV-1 gene expression: Nuclear retention of HBZ mRNAs. Blood, 117(18), 4855-4859. https://doi.org/10.1182/blood-2010-11-316463

Kinetics and intracellular compartmentalization of HTLV-1 gene expression : Nuclear retention of HBZ mRNAs. / Rende, Francesca; Cavallari, Ilaria; Corradin, Alberto; Silic-Benussi, Micol; Toulza, Frederic; Toffolo, Gianna M.; Tanaka, Yuetsu; Jacobson, Steven; Taylor, Graham P.; D'Agostino, Donna M.; Bangham, Charles R M; Ciminale, Vincenzo.

In: Blood, Vol. 117, No. 18, 05.05.2011, p. 4855-4859.

Research output: Contribution to journalArticle

Rende, F, Cavallari, I, Corradin, A, Silic-Benussi, M, Toulza, F, Toffolo, GM, Tanaka, Y, Jacobson, S, Taylor, GP, D'Agostino, DM, Bangham, CRM & Ciminale, V 2011, 'Kinetics and intracellular compartmentalization of HTLV-1 gene expression: Nuclear retention of HBZ mRNAs', Blood, vol. 117, no. 18, pp. 4855-4859. https://doi.org/10.1182/blood-2010-11-316463
Rende F, Cavallari I, Corradin A, Silic-Benussi M, Toulza F, Toffolo GM et al. Kinetics and intracellular compartmentalization of HTLV-1 gene expression: Nuclear retention of HBZ mRNAs. Blood. 2011 May 5;117(18):4855-4859. https://doi.org/10.1182/blood-2010-11-316463
Rende, Francesca ; Cavallari, Ilaria ; Corradin, Alberto ; Silic-Benussi, Micol ; Toulza, Frederic ; Toffolo, Gianna M. ; Tanaka, Yuetsu ; Jacobson, Steven ; Taylor, Graham P. ; D'Agostino, Donna M. ; Bangham, Charles R M ; Ciminale, Vincenzo. / Kinetics and intracellular compartmentalization of HTLV-1 gene expression : Nuclear retention of HBZ mRNAs. In: Blood. 2011 ; Vol. 117, No. 18. pp. 4855-4859.
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