Kinetics features of ascorbic acid oxidase after partial deglycation

G. D'Andrea, M. Maccarrone, A. Oratore, L. Avigliano, A. Messerschmidt

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

By means of specific exoglycosidases, sugars have been removed under non-denaturing conditions from ascorbic acid oxidase (AAO), different deglycation schedules being followed. Our results indicate that deglycation clearly affects the kinetic features of AAO, leading to an increase of 'affinity' and 'catalytic ability' of the enzymic forms so generated. A better exposure of the catalytic-site residues could be supposed to occur upon treatment with exoglycosidases. This is supported by the three-dimensional X-ray structure of zucchini (Cucurbita pepo medullosa; courgette) AAO.

Original languageEnglish
Pages (from-to)601-604
Number of pages4
JournalBiochemical Journal
Volume264
Issue number2
Publication statusPublished - 1989

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Ascorbic Acid
Oxidoreductases
Glycoside Hydrolases
Kinetics
Cucurbita
Sugars
Catalytic Domain
Appointments and Schedules
X-Rays
X rays

ASJC Scopus subject areas

  • Biochemistry

Cite this

D'Andrea, G., Maccarrone, M., Oratore, A., Avigliano, L., & Messerschmidt, A. (1989). Kinetics features of ascorbic acid oxidase after partial deglycation. Biochemical Journal, 264(2), 601-604.

Kinetics features of ascorbic acid oxidase after partial deglycation. / D'Andrea, G.; Maccarrone, M.; Oratore, A.; Avigliano, L.; Messerschmidt, A.

In: Biochemical Journal, Vol. 264, No. 2, 1989, p. 601-604.

Research output: Contribution to journalArticle

D'Andrea, G, Maccarrone, M, Oratore, A, Avigliano, L & Messerschmidt, A 1989, 'Kinetics features of ascorbic acid oxidase after partial deglycation', Biochemical Journal, vol. 264, no. 2, pp. 601-604.
D'Andrea G, Maccarrone M, Oratore A, Avigliano L, Messerschmidt A. Kinetics features of ascorbic acid oxidase after partial deglycation. Biochemical Journal. 1989;264(2):601-604.
D'Andrea, G. ; Maccarrone, M. ; Oratore, A. ; Avigliano, L. ; Messerschmidt, A. / Kinetics features of ascorbic acid oxidase after partial deglycation. In: Biochemical Journal. 1989 ; Vol. 264, No. 2. pp. 601-604.
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