Abstract
The preparation of bacterial intermediates bearing complement components at various steps of the complement sequence was investigated by suspending immunoglobulin M-opsonized Escherichia coli O111:B4 cells in complement-deficient sera at different temperatures and ionic strengths. The optimal conditions for the formation of the intermediates at T(max) were found to be an ionic strength of 0.091 μ and a temperature of 37°C, except for BAC142, which could be formed equally well at room temperature. In contrast to all the other intermediates, which, once formed at T(max), were stable in the presence of the whole serum, BAC142 decayed with a half-life of 10 min due to the lability of bound C2. Washing with a buffer of either 0.091 or 0.046 μ did not affect the bacterial intermediates, with the exception of BAC1-3 formed either in the presence of C5-deficient serum or with purified C3 added to BAC142. All the intermediates were found to be stable after incubation in 0.091-μ buffer for 30 min at 37°C.
| Original language | English |
|---|---|
| Pages (from-to) | 402-406 |
| Number of pages | 5 |
| Journal | Infection and Immunity |
| Volume | 49 |
| Issue number | 2 |
| Publication status | Published - 1985 |
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ASJC Scopus subject areas
- Immunology
Cite this
Kinetics of assembly and decay of complement components on Escherichia coli O111 : B4 preparation of stable intermediates. / Rottini, G.; Tedesco, F.; Basaglia, M.; Roncelli, L.; Patriarca, P.
In: Infection and Immunity, Vol. 49, No. 2, 1985, p. 402-406.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Kinetics of assembly and decay of complement components on Escherichia coli O111
T2 - B4 preparation of stable intermediates
AU - Rottini, G.
AU - Tedesco, F.
AU - Basaglia, M.
AU - Roncelli, L.
AU - Patriarca, P.
PY - 1985
Y1 - 1985
N2 - The preparation of bacterial intermediates bearing complement components at various steps of the complement sequence was investigated by suspending immunoglobulin M-opsonized Escherichia coli O111:B4 cells in complement-deficient sera at different temperatures and ionic strengths. The optimal conditions for the formation of the intermediates at T(max) were found to be an ionic strength of 0.091 μ and a temperature of 37°C, except for BAC142, which could be formed equally well at room temperature. In contrast to all the other intermediates, which, once formed at T(max), were stable in the presence of the whole serum, BAC142 decayed with a half-life of 10 min due to the lability of bound C2. Washing with a buffer of either 0.091 or 0.046 μ did not affect the bacterial intermediates, with the exception of BAC1-3 formed either in the presence of C5-deficient serum or with purified C3 added to BAC142. All the intermediates were found to be stable after incubation in 0.091-μ buffer for 30 min at 37°C.
AB - The preparation of bacterial intermediates bearing complement components at various steps of the complement sequence was investigated by suspending immunoglobulin M-opsonized Escherichia coli O111:B4 cells in complement-deficient sera at different temperatures and ionic strengths. The optimal conditions for the formation of the intermediates at T(max) were found to be an ionic strength of 0.091 μ and a temperature of 37°C, except for BAC142, which could be formed equally well at room temperature. In contrast to all the other intermediates, which, once formed at T(max), were stable in the presence of the whole serum, BAC142 decayed with a half-life of 10 min due to the lability of bound C2. Washing with a buffer of either 0.091 or 0.046 μ did not affect the bacterial intermediates, with the exception of BAC1-3 formed either in the presence of C5-deficient serum or with purified C3 added to BAC142. All the intermediates were found to be stable after incubation in 0.091-μ buffer for 30 min at 37°C.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021863673&partnerID=8YFLogxK
M3 - Article
C2 - 3894235
AN - SCOPUS:0021863673
VL - 49
SP - 402
EP - 406
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 2
ER -