Kinetics of caffeine metabolism in control and 3-methylcholanthrene induced rat liver microsomes

M. Bonati; A. Celardo; F. Galletti; R. Latini; F. Tursi; G. Belvedere

doi:10.1016/0378-4274(84)90223-6

Kinetics of caffeine metabolism in control and 3-methylcholanthrene induced rat liver microsomes

M. Bonati, A. Celardo, F. Galletti, R. Latini, F. Tursi, G. Belvedere

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article › peer-review

Abstract

The kinetics of formation of primary metabolites of caffeine (paraxanthine, theophylline, theobromine and 1,3,7-trimethyluric acid) was studied in control (CO) and 3-methylcholanthrene-induced (MC) rat liver microsomes. V_max was similar but K_m was 16 times lower for total caffeine metabolism in CO and MC microsomes, respectively. Similar behaviour was observed in the formation of each metabolite. Single metabolites showed different degrees of induction at non-saturating concentrations of caffeine. Kinetics was non-linear in CO microsomes.

Original language	English
Pages (from-to)	53-58
Number of pages	6
Journal	Toxicology Letters
Volume	21
Issue number	1
DOIs	https://doi.org/10.1016/0378-4274(84)90223-6
Publication status	Published - 1984

Keywords

3-methylcholanthrene
Caffeine metabolism
K
microsomes
rats
V

ASJC Scopus subject areas

Toxicology

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10.1016/0378-4274(84)90223-6

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abstract = "The kinetics of formation of primary metabolites of caffeine (paraxanthine, theophylline, theobromine and 1,3,7-trimethyluric acid) was studied in control (CO) and 3-methylcholanthrene-induced (MC) rat liver microsomes. Vmax was similar but Km was 16 times lower for total caffeine metabolism in CO and MC microsomes, respectively. Similar behaviour was observed in the formation of each metabolite. Single metabolites showed different degrees of induction at non-saturating concentrations of caffeine. Kinetics was non-linear in CO microsomes.",

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year = "1984",

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T1 - Kinetics of caffeine metabolism in control and 3-methylcholanthrene induced rat liver microsomes

AU - Bonati, M.

AU - Celardo, A.

AU - Galletti, F.

AU - Latini, R.

AU - Tursi, F.

AU - Belvedere, G.

PY - 1984

Y1 - 1984

N2 - The kinetics of formation of primary metabolites of caffeine (paraxanthine, theophylline, theobromine and 1,3,7-trimethyluric acid) was studied in control (CO) and 3-methylcholanthrene-induced (MC) rat liver microsomes. Vmax was similar but Km was 16 times lower for total caffeine metabolism in CO and MC microsomes, respectively. Similar behaviour was observed in the formation of each metabolite. Single metabolites showed different degrees of induction at non-saturating concentrations of caffeine. Kinetics was non-linear in CO microsomes.

AB - The kinetics of formation of primary metabolites of caffeine (paraxanthine, theophylline, theobromine and 1,3,7-trimethyluric acid) was studied in control (CO) and 3-methylcholanthrene-induced (MC) rat liver microsomes. Vmax was similar but Km was 16 times lower for total caffeine metabolism in CO and MC microsomes, respectively. Similar behaviour was observed in the formation of each metabolite. Single metabolites showed different degrees of induction at non-saturating concentrations of caffeine. Kinetics was non-linear in CO microsomes.

KW - 3-methylcholanthrene

KW - Caffeine metabolism

KW - K

KW - microsomes

KW - rats

KW - V

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Kinetics of caffeine metabolism in control and 3-methylcholanthrene induced rat liver microsomes

Abstract

Keywords

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