Kinetics of the use of cryopreserved autologous stem cell grafts: A GITMO-SIDEM survey

Jacopo Olivieri, Luca Pierelli, Martino Introna, Patrizia Accorsi, Alberto Bosi, Paolo Perseghin, Marco Risso, Annino Pandolfi, Stefania Mancini, Monia Marchetti, Simone Dal Pozzo, Elisa Gotti, Alessandro Rambaldi, Pietro Leoni, Attilio Olivieri

Research output: Contribution to journalArticlepeer-review

Abstract

Background aims: Hematopoietic stem cell cryopreservation significantly contributed to autologous stem cell transplantation (ASCT). Cryopreserved stem cell units (SCU) are expected to be used soon after harvesting for most purposes, but, in a number of cases, they remain stored for some time, creating an increasing load for SCU depositories. Disposal policies vary widely in each center, and the existing guidelines are insufficient. Methods: We conducted a survey of seven Gruppo Italiano Trapianto di Midollo Osseo centers to investigate the outcome of SCU harvested from January 2005 to December 2009 for ASCT. The data from 1603 collections were gathered, for a total of 5822 SCU. Results: In our cohort, 79% of patients collected >5× 106 CD34+ cells/kg, and 3.4% collected6 CD34+ cells/kg. Up to 21% of all the patients and 42% of those with acute leukemia did not undergo reinfusion, and 37% of the cryopreserved SCU were excess, resulting from patients not reinfusing or partially reinfusing. Less than one-third of the excess SCU was disposed, and the major causes of disposal were death and, in a minority of cases, withdrawal of the indication for ASCT. In our analysis, very few first reinfusions occurred after 2 years, and those after 5 years were exceptional. Through the use of a multivariate analysis, we sought to identify the risk factors for collection non-use, independent of the centers' policies. Non-use of SCU was significantly associated with patients with acute leukemia, collections of6 CD34/kg and lower age groups. Conclusions: These dataserve as a valid basis to support rational recommendations for cost-effective storage and disposal of SCU.

Original languageEnglish
Pages (from-to)101-110
Number of pages10
JournalCytotherapy
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 2014

Keywords

  • Biological specimen banks
  • Cryopreservation
  • Hematopoietic progenitor cells
  • Hematopoietic stem cell transplantation
  • Long-term storage
  • Medical waste disposal

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research
  • Transplantation
  • Genetics(clinical)
  • Oncology
  • Immunology and Allergy
  • Immunology

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