KSRP and MicroRNA 145 are negative regulators of lipolysis in white adipose tissue

Yi Yu Lin, Chu Fang Chou, Matteo Giovarelli, Paola Briata, Roberto Gherzi, Ching Yi Chen

Research output: Contribution to journalArticlepeer-review

Abstract

White adipose tissue (WAT) releases fatty acids from stored triacylglycerol for an energy source. Here, we report that targeted deletion of KH-type splicing regulatory protein (KSRP), an RNA-binding protein that regulates gene expression at multiple levels, enhances lipolysis in epididymal WAT (eWAT) because of the upregulation of genes promoting lipolytic activity. Expression of microRNA 145 (miR-145) is decreased because of impaired primary miR-145 processing in Ksrp-/- eWAT. We show that miR-145 directly targets and represses Foxo1 and Cgi58, activators of lipolytic activity, and forced expression of miR-145 attenuates lipolysis. This study reveals a novel in vivo function of KSRP in controlling adipose lipolysis through posttranscriptional regulation of miR-145 expression.

Original languageEnglish
Pages (from-to)2339-2349
Number of pages11
JournalMolecular and Cellular Biology
Volume34
Issue number12
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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