The effects of L-α-glycerylphosphorylcholine (α-GPC) on scopolamine-induced memory impairment and on brain acetylcholine (ACh) synthesis and release were investigated in rats. Oral administration of α-GPC 3 h before the behavioural test prevented the learning impairment induced by scopalamine given 30 min before the acquisition of a passive avoidance response. Similarly, retrograde amnesia induced by scopalamine, given immediately after acquisition training, was also completely reversed by the drug. These effects were dose-dependent with a maximum at 300 mg/kg. The mechanism of action of this compound was investigated by measuring hippocampal ACh synthesis and release both in vivo by means of the microdialysis technique and in vitro in tissue slices. α-GPC dose dependently increased ACh release with a maximum ast 300 mg/kg. In addition, i.v. injection of [14α-GPC resulted in [14C]ACh formation. The data suggest that the behavioural effects of αGPC may be related to its property to increase hippocampal ACh synthesis and release.
- Acetylcholine release
- Choline precursors
- Passice avoidance
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience