L-α-glycerylphorylcholine antagonizes scopolamine-induced amnesia and enhances hippocampal cholinergic transmission in the rat

Sandra Sigala, Assunta Imperato, Paola Rizzonelli, Paola Casolini, Cristina Missale, PierFranco Spano

Research output: Contribution to journalArticle

Abstract

The effects of L-α-glycerylphosphorylcholine (α-GPC) on scopolamine-induced memory impairment and on brain acetylcholine (ACh) synthesis and release were investigated in rats. Oral administration of α-GPC 3 h before the behavioural test prevented the learning impairment induced by scopalamine given 30 min before the acquisition of a passive avoidance response. Similarly, retrograde amnesia induced by scopalamine, given immediately after acquisition training, was also completely reversed by the drug. These effects were dose-dependent with a maximum at 300 mg/kg. The mechanism of action of this compound was investigated by measuring hippocampal ACh synthesis and release both in vivo by means of the microdialysis technique and in vitro in tissue slices. α-GPC dose dependently increased ACh release with a maximum ast 300 mg/kg. In addition, i.v. injection of [14α-GPC resulted in [14C]ACh formation. The data suggest that the behavioural effects of αGPC may be related to its property to increase hippocampal ACh synthesis and release.

Original languageEnglish
Pages (from-to)351-358
Number of pages8
JournalEuropean Journal of Pharmacology
Volume211
Issue number3
DOIs
Publication statusPublished - Feb 18 1992

Keywords

  • Acetylcholine
  • Acetylcholine release
  • Choline precursors
  • Hippocampus
  • Passice avoidance
  • Scopolamine

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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