L-Carnitine activates calcium signaling in human osteoblasts

A Ferraretto, M Bottani, I Villa, L Giusto, M Signo, P Senesi, A Montesano, F Vacante, L Luzi, A Rubinacci, I Terruzzi

Research output: Contribution to journalArticle

Abstract

L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity. © 2018 Elsevier Ltd
Original languageEnglish
Pages (from-to)270-278
Number of pages9
JournalJournal of Functional Foods
Volume47
Issue number4
DOIs
Publication statusPublished - 2018

Fingerprint

Calcium Signaling
Carnitine
carnitine
osteoblasts
Osteoblasts
calcium
Calcium
Calcium-Sensing Receptors
Cell Differentiation
cell proliferation
Cell Proliferation
L-Type Calcium Channels
receptors
calcium channels
phospholipase C
Fura-2
Type C Phospholipases
calcium signaling
cell differentiation
Endoplasmic Reticulum

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L-Carnitine activates calcium signaling in human osteoblasts. / Ferraretto, A; Bottani, M; Villa, I; Giusto, L; Signo, M; Senesi, P; Montesano, A; Vacante, F; Luzi, L; Rubinacci, A; Terruzzi, I.

In: Journal of Functional Foods, Vol. 47, No. 4, 2018, p. 270-278.

Research output: Contribution to journalArticle

Ferraretto, A, Bottani, M, Villa, I, Giusto, L, Signo, M, Senesi, P, Montesano, A, Vacante, F, Luzi, L, Rubinacci, A & Terruzzi, I 2018, 'L-Carnitine activates calcium signaling in human osteoblasts', Journal of Functional Foods, vol. 47, no. 4, pp. 270-278. https://doi.org/10.1016/j.jff.2018.05.068
Ferraretto, A ; Bottani, M ; Villa, I ; Giusto, L ; Signo, M ; Senesi, P ; Montesano, A ; Vacante, F ; Luzi, L ; Rubinacci, A ; Terruzzi, I. / L-Carnitine activates calcium signaling in human osteoblasts. In: Journal of Functional Foods. 2018 ; Vol. 47, No. 4. pp. 270-278.
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AU - Bottani, M

AU - Villa, I

AU - Giusto, L

AU - Signo, M

AU - Senesi, P

AU - Montesano, A

AU - Vacante, F

AU - Luzi, L

AU - Rubinacci, A

AU - Terruzzi, I

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AB - L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity. © 2018 Elsevier Ltd

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