L-Carnitine activates calcium signaling in human osteoblasts

A Ferraretto, M Bottani, I Villa, L Giusto, M Signo, P Senesi, A Montesano, F Vacante, L Luzi, A Rubinacci, I Terruzzi

Research output: Contribution to journalArticle

Abstract

L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity. © 2018 Elsevier Ltd
Original languageEnglish
Pages (from-to)270-278
Number of pages9
JournalJournal of Functional Foods
Volume47
Issue number4
DOIs
Publication statusPublished - 2018

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    Ferraretto, A., Bottani, M., Villa, I., Giusto, L., Signo, M., Senesi, P., Montesano, A., Vacante, F., Luzi, L., Rubinacci, A., & Terruzzi, I. (2018). L-Carnitine activates calcium signaling in human osteoblasts. Journal of Functional Foods, 47(4), 270-278. https://doi.org/10.1016/j.jff.2018.05.068