L-Carnitine activates calcium signaling in human osteoblasts

A Ferraretto; M Bottani; I Villa; L Giusto; M Signo; P Senesi; A Montesano; F Vacante; L Luzi; A Rubinacci; I Terruzzi

doi:10.1016/j.jff.2018.05.068

L-Carnitine activates calcium signaling in human osteoblasts

A Ferraretto, M Bottani, I Villa, L Giusto, M Signo, P Senesi, A Montesano, F Vacante, L Luzi, A Rubinacci, I Terruzzi

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity. © 2018 Elsevier Ltd

Original language	English
Pages (from-to)	270-278
Number of pages	9
Journal	Journal of Functional Foods
Volume	47
Issue number	4
DOIs	https://doi.org/10.1016/j.jff.2018.05.068
Publication status	Published - 2018

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title = "L-Carnitine activates calcium signaling in human osteoblasts",

abstract = "L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity. {\textcopyright} 2018 Elsevier Ltd",

author = "A Ferraretto and M Bottani and I Villa and L Giusto and M Signo and P Senesi and A Montesano and F Vacante and L Luzi and A Rubinacci and I Terruzzi",

year = "2018",

doi = "10.1016/j.jff.2018.05.068",

language = "English",

volume = "47",

pages = "270--278",

journal = "Journal of Functional Foods",

issn = "1756-4646",

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T1 - L-Carnitine activates calcium signaling in human osteoblasts

AU - Ferraretto, A

AU - Bottani, M

AU - Villa, I

AU - Giusto, L

AU - Signo, M

AU - Senesi, P

AU - Montesano, A

AU - Vacante, F

AU - Luzi, L

AU - Rubinacci, A

AU - Terruzzi, I

PY - 2018

Y1 - 2018

N2 - L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity. © 2018 Elsevier Ltd

AB - L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity. © 2018 Elsevier Ltd

U2 - 10.1016/j.jff.2018.05.068

DO - 10.1016/j.jff.2018.05.068

M3 - Article

VL - 47

SP - 270

EP - 278

JO - Journal of Functional Foods

JF - Journal of Functional Foods

SN - 1756-4646

IS - 4

ER -