Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 270-278 |
| Number of pages | 9 |
| Journal | Journal of Functional Foods |
| Volume | 47 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2018 |
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L-Carnitine activates calcium signaling in human osteoblasts. / Ferraretto, A; Bottani, M; Villa, I; Giusto, L; Signo, M; Senesi, P; Montesano, A; Vacante, F; Luzi, L; Rubinacci, A; Terruzzi, I.
In: Journal of Functional Foods, Vol. 47, No. 4, 2018, p. 270-278.Research output: Contribution to journal › Article
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TY - JOUR
T1 - L-Carnitine activates calcium signaling in human osteoblasts
AU - Ferraretto, A
AU - Bottani, M
AU - Villa, I
AU - Giusto, L
AU - Signo, M
AU - Senesi, P
AU - Montesano, A
AU - Vacante, F
AU - Luzi, L
AU - Rubinacci, A
AU - Terruzzi, I
PY - 2018
Y1 - 2018
N2 - L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity. © 2018 Elsevier Ltd
AB - L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity. © 2018 Elsevier Ltd
U2 - 10.1016/j.jff.2018.05.068
DO - 10.1016/j.jff.2018.05.068
M3 - Article
VL - 47
SP - 270
EP - 278
JO - Journal of Functional Foods
JF - Journal of Functional Foods
SN - 1756-4646
IS - 4
ER -