L-Carnitine activates calcium signaling in human osteoblasts

Anita Ferraretto, Michela Bottani, Isabella Villa, Lucia Giusto, Michela Signo, Pamela Senesi, Anna Montesano, Fernanda Vacante, Livio Luzi, Alessandro Rubinacci, Ileana Terruzzi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity.

Original languageEnglish
Pages (from-to)270-278
Number of pages9
JournalJournal of Functional Foods
Volume47
DOIs
Publication statusPublished - Aug 1 2018

Fingerprint

Calcium Signaling
Carnitine
carnitine
osteoblasts
Osteoblasts
calcium
Calcium
Calcium-Sensing Receptors
Cell Differentiation
cell proliferation
Cell Proliferation
L-Type Calcium Channels
receptors
calcium channels
phospholipase C
Fura-2
Type C Phospholipases
calcium signaling
cell differentiation
Endoplasmic Reticulum

Keywords

  • Calcium sensing receptor
  • Human primary osteoblast-like cells
  • L-Carnitine
  • L-VDCC
  • Mitochondrial calcium stores
  • PLC sensitive stores

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

L-Carnitine activates calcium signaling in human osteoblasts. / Ferraretto, Anita; Bottani, Michela; Villa, Isabella; Giusto, Lucia; Signo, Michela; Senesi, Pamela; Montesano, Anna; Vacante, Fernanda; Luzi, Livio; Rubinacci, Alessandro; Terruzzi, Ileana.

In: Journal of Functional Foods, Vol. 47, 01.08.2018, p. 270-278.

Research output: Contribution to journalArticle

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AU - Senesi, Pamela

AU - Montesano, Anna

AU - Vacante, Fernanda

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AU - Rubinacci, Alessandro

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