l-Carnitine uptake in differentiating human cultured muscle

A. Martinnzzi, L. Vergani, M. Rosa, C. Angelini

Research output: Contribution to journalArticlepeer-review

Abstract

We studied carnitine uptake in human skeletal muscle growing in culture for up to 30 days, and correlated it to the degree of muscle differentiation revealed by myotube formation and muscle-specific creatine-kinase isozyme accumulation. In our study carnitine uptake was a saturable specific process with two distinct components: a high affinity uptake at carnitine concentration between 0.5 and 10 μM and a low affinity uptake at carnitine concentration between 25 and 200 μM. High affinity uptake (Km 4.17-5.50 μM, Vmax 11.78-19.6 pmol/h per mg protein) did not change during muscle maturation in culture. Low affinity uptake showed significant changes in Km and Vmax in the various stages of muscle differentiation. Our studies suggest the existence of a muscle-specific system, operating at physiological carnitine concentration, which gradually developes during muscle maturation in culture. We hypothesize that a defect of the low affinity muscle-specific uptake might be the cause of the primary muscle carnitine deficiency syndrome.

Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1095
Issue number3
DOIs
Publication statusPublished - Nov 12 1991

Keywords

  • Cell differentiatian
  • Fibroblast
  • l-Carnitine
  • Myoblast
  • Myotube
  • Skeletal muscle

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

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