Label-free and fluorescence photonic crystal biochips for early cancer biomarker detection

A. Sinibaldi, N. Danz, E. Sepe, P. Munzert, A. Occhicone, M. Allegretti, E. Giordani, P. Giacomini, F. Michelotti

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report on the development of a biosensing platform that combines label-free and fluorescence based detection on disposable Bloch surface wave biochips. This system is applied to the detection of the HER2-neu/ErbB2 clinical biomarker related to breast cancer development. We first describe the design and fabrication of the BSW biochips as well as the principle of operation of the optical reading instrument. Then, the approaches for surface functionalization and immobilization of proteins for specific detection on the biochips are discussed. Finally, experimental results on a sandwich immunoassay for ErbB2 detection in cell lysates are presented.

Original languageEnglish
Title of host publicationFrontiers in Biological Detection
Subtitle of host publicationFrom Nanosensors to Systems XI
EditorsAmos Danielli, Benjamin L. Miller, Sharon M. Weiss
PublisherSPIE
ISBN (Electronic)9781510624320
DOIs
Publication statusPublished - Jan 1 2019
EventFrontiers in Biological Detection: From Nanosensors to Systems XI 2019 - San Francisco, United States
Duration: Feb 3 2019Feb 4 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10895
ISSN (Print)1605-7422

Conference

ConferenceFrontiers in Biological Detection: From Nanosensors to Systems XI 2019
Country/TerritoryUnited States
CitySan Francisco
Period2/3/192/4/19

Keywords

  • Biophotonics
  • Bloch surface waves
  • Label-free biosensors
  • Optical biosensing
  • Ovalbumin
  • Photonic crystals

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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