Label free monitoring of megakaryocytic development and proplatelet formation in vitro

Dimitra Pouli, Lorenzo Tozzi, Carlo A. Alonzo, Zhiyi Liu, David L. Kaplan, Alessandra Balduini, Irene Georgakoudi

Research output: Contribution to journalArticlepeer-review


Megakaryopoiesis and platelet production are complex biological processes that require tight regulation of successive lineage commitment steps and are ultimately responsible for maintaining and renewing the pool of circulating platelets in the blood. Despite major advancements in the understanding of megakaryocytic biology, the detailed mechanisms driving megakaryocytic differentiation have yet to be elucidated. Here we show that automated image analysis algorithms applied to two-photon excited fluorescence (TPEF) images can non-invasively monitor structural and metabolic megakaryocyte behavior changes occurring during differentiation and platelet formation in vitro. Our results demonstrate that high-contrast, label-free two photon imaging holds great potential in studying the underlying physiological processes controlling the intricate process of platelet production.

Original languageEnglish
Article number#300649
Pages (from-to)4742-4755
Number of pages14
JournalBiomedical Optics Express
Issue number10
Publication statusPublished - Oct 1 2017


  • (170.0170) medical optics and biotechnology
  • (170.1470) blood or tissue constituent monitoring
  • (170.3880) medical and biological imaging
  • (180.2520) fluorescence microscopy
  • (190.0190) nonlinear optics

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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