Label-free monitoring of tissue biochemistry following traumatic brain injury using Raman spectroscopy

Jakub Maciej Surmacki, Laura Ansel-Bollepalli, Francesca Pischiutta, Elisa R. Zanier, Ari Ercole, Sarah Elizabeth Bohndiek

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Traumatic brain injury (TBI) constitutes a major cause of death and long-term disability. At present, we lack methods to non-invasively track tissue biochemistry and hence select appropriate interventions for patients. We hypothesized that detailed label-free vibrational chemical analysis of focal TBI could provide such information. We assessed the early spatial and temporal changes in tissue biochemistry that are associated with brain injury in mice. Numerous differences were observed in the spectra of the contusion core and pericontusional tissue between 2 and 7 days. For example, a strong signal from haem was seen in the contusion core at 2 days due to haemorrhage, which subsequently resolved. More importantly, elevated cholesterol levels were demonstrated by 7 days, which may be a marker of important cell repair processes. Principal component analysis revealed an early 'acute' component dominated by haemorrhage and a delayed component reflecting changes in protein and lipid composition. Notably we demonstrated changes in Raman signature with time even in the contralateral hemisphere when compared to sham control mice. Raman spectroscopy therefore shows promise as a probe that is sensitive to important pathobiological processes in TBI and could be applied in future both in the experimental setting, as well as in the clinic.

Original languageEnglish
Pages (from-to)132-139
Number of pages8
JournalAnalyst
Volume142
Issue number1
DOIs
Publication statusPublished - Jan 7 2017

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Biochemistry
Raman Spectrum Analysis
biochemistry
Raman spectroscopy
brain
Labels
Brain
Contusions
Tissue
Brain Injuries
Monitoring
monitoring
Hemorrhage
Hypercholesterolemia
Principal Component Analysis
Heme
cause of death
Cause of Death
Cholesterol
disability

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Surmacki, J. M., Ansel-Bollepalli, L., Pischiutta, F., Zanier, E. R., Ercole, A., & Bohndiek, S. E. (2017). Label-free monitoring of tissue biochemistry following traumatic brain injury using Raman spectroscopy. Analyst, 142(1), 132-139. https://doi.org/10.1039/c6an02238c

Label-free monitoring of tissue biochemistry following traumatic brain injury using Raman spectroscopy. / Surmacki, Jakub Maciej; Ansel-Bollepalli, Laura; Pischiutta, Francesca; Zanier, Elisa R.; Ercole, Ari; Bohndiek, Sarah Elizabeth.

In: Analyst, Vol. 142, No. 1, 07.01.2017, p. 132-139.

Research output: Contribution to journalArticle

Surmacki, JM, Ansel-Bollepalli, L, Pischiutta, F, Zanier, ER, Ercole, A & Bohndiek, SE 2017, 'Label-free monitoring of tissue biochemistry following traumatic brain injury using Raman spectroscopy', Analyst, vol. 142, no. 1, pp. 132-139. https://doi.org/10.1039/c6an02238c
Surmacki, Jakub Maciej ; Ansel-Bollepalli, Laura ; Pischiutta, Francesca ; Zanier, Elisa R. ; Ercole, Ari ; Bohndiek, Sarah Elizabeth. / Label-free monitoring of tissue biochemistry following traumatic brain injury using Raman spectroscopy. In: Analyst. 2017 ; Vol. 142, No. 1. pp. 132-139.
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