Labeling protocols for in vivo tracking of human skeletal muscle cells (HSkMCs) by magnetic resonance and bioluminescence imaging

Ilaria V. Libani, Giovanni Lucignani, Umberto Gianelli, Anna Degrassi, Micaela Russo, Silvano Bosari, Mario Clerici, Luisa Ottobrini

Research output: Contribution to journalArticle

Abstract

Purpose: We propose herein labeling protocols for multimodal in vivo visualization of human skeletal muscle cells (HSkMCs) by MRI and BLI to investigate the survival, localization, and proliferation/differentiation of these cells in cell-mediated therapy. Procedures: HSkMCs were labeled with different quantities of Endorem® and transfection agents or infected with lentiviral vector expressing the luciferase gene under the myogenin promoter. Cells were evaluated before and after intra-arterial injection in NUDE mice with N 2-induced muscle inflammation. Results: Neither iron labeling nor infection affected cell features; the number of iron-positive cells increased proportionally to the iron content in the medium and in the presence of transfection agents. Loaded cells were detected for up to 1 month by MRI and 2 months by BLI. Conclusions: These protocols could be used to visualize new stem cells, in vivo and over time, in preclinical studies of cell-based treatments for myopathies of different etiologies.

Original languageEnglish
Pages (from-to)47-59
Number of pages13
JournalMolecular Imaging and Biology
Volume14
Issue number1
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Muscle Cells
Skeletal Muscle
Magnetic Resonance Imaging
Iron
Transfection
Myogenin
Intra-Arterial Injections
Muscular Diseases
Cell- and Tissue-Based Therapy
Luciferases
Cell Differentiation
Stem Cells
Cell Proliferation
Inflammation
Muscles
Survival
Infection
Genes
Therapeutics

Keywords

  • Bioluminescence imaging
  • Cell tracking
  • Human skeletal muscle cells
  • Magnetic resonance imaging
  • Superparamagnetic iron oxide
  • Transplantation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging

Cite this

Labeling protocols for in vivo tracking of human skeletal muscle cells (HSkMCs) by magnetic resonance and bioluminescence imaging. / Libani, Ilaria V.; Lucignani, Giovanni; Gianelli, Umberto; Degrassi, Anna; Russo, Micaela; Bosari, Silvano; Clerici, Mario; Ottobrini, Luisa.

In: Molecular Imaging and Biology, Vol. 14, No. 1, 02.2012, p. 47-59.

Research output: Contribution to journalArticle

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AU - Degrassi, Anna

AU - Russo, Micaela

AU - Bosari, Silvano

AU - Clerici, Mario

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