Abstract
Purpose: We propose herein labeling protocols for multimodal in vivo visualization of human skeletal muscle cells (HSkMCs) by MRI and BLI to investigate the survival, localization, and proliferation/differentiation of these cells in cell-mediated therapy. Procedures: HSkMCs were labeled with different quantities of Endorem® and transfection agents or infected with lentiviral vector expressing the luciferase gene under the myogenin promoter. Cells were evaluated before and after intra-arterial injection in NUDE mice with N 2-induced muscle inflammation. Results: Neither iron labeling nor infection affected cell features; the number of iron-positive cells increased proportionally to the iron content in the medium and in the presence of transfection agents. Loaded cells were detected for up to 1 month by MRI and 2 months by BLI. Conclusions: These protocols could be used to visualize new stem cells, in vivo and over time, in preclinical studies of cell-based treatments for myopathies of different etiologies.
| Original language | English |
|---|---|
| Pages (from-to) | 47-59 |
| Number of pages | 13 |
| Journal | Molecular Imaging and Biology |
| Volume | 14 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 2012 |
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Keywords
- Bioluminescence imaging
- Cell tracking
- Human skeletal muscle cells
- Magnetic resonance imaging
- Superparamagnetic iron oxide
- Transplantation
ASJC Scopus subject areas
- Cancer Research
- Oncology
- Radiology Nuclear Medicine and imaging
Cite this
Labeling protocols for in vivo tracking of human skeletal muscle cells (HSkMCs) by magnetic resonance and bioluminescence imaging. / Libani, Ilaria V.; Lucignani, Giovanni; Gianelli, Umberto; Degrassi, Anna; Russo, Micaela; Bosari, Silvano; Clerici, Mario; Ottobrini, Luisa.
In: Molecular Imaging and Biology, Vol. 14, No. 1, 02.2012, p. 47-59.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Labeling protocols for in vivo tracking of human skeletal muscle cells (HSkMCs) by magnetic resonance and bioluminescence imaging
AU - Libani, Ilaria V.
AU - Lucignani, Giovanni
AU - Gianelli, Umberto
AU - Degrassi, Anna
AU - Russo, Micaela
AU - Bosari, Silvano
AU - Clerici, Mario
AU - Ottobrini, Luisa
PY - 2012/2
Y1 - 2012/2
N2 - Purpose: We propose herein labeling protocols for multimodal in vivo visualization of human skeletal muscle cells (HSkMCs) by MRI and BLI to investigate the survival, localization, and proliferation/differentiation of these cells in cell-mediated therapy. Procedures: HSkMCs were labeled with different quantities of Endorem® and transfection agents or infected with lentiviral vector expressing the luciferase gene under the myogenin promoter. Cells were evaluated before and after intra-arterial injection in NUDE mice with N 2-induced muscle inflammation. Results: Neither iron labeling nor infection affected cell features; the number of iron-positive cells increased proportionally to the iron content in the medium and in the presence of transfection agents. Loaded cells were detected for up to 1 month by MRI and 2 months by BLI. Conclusions: These protocols could be used to visualize new stem cells, in vivo and over time, in preclinical studies of cell-based treatments for myopathies of different etiologies.
AB - Purpose: We propose herein labeling protocols for multimodal in vivo visualization of human skeletal muscle cells (HSkMCs) by MRI and BLI to investigate the survival, localization, and proliferation/differentiation of these cells in cell-mediated therapy. Procedures: HSkMCs were labeled with different quantities of Endorem® and transfection agents or infected with lentiviral vector expressing the luciferase gene under the myogenin promoter. Cells were evaluated before and after intra-arterial injection in NUDE mice with N 2-induced muscle inflammation. Results: Neither iron labeling nor infection affected cell features; the number of iron-positive cells increased proportionally to the iron content in the medium and in the presence of transfection agents. Loaded cells were detected for up to 1 month by MRI and 2 months by BLI. Conclusions: These protocols could be used to visualize new stem cells, in vivo and over time, in preclinical studies of cell-based treatments for myopathies of different etiologies.
KW - Bioluminescence imaging
KW - Cell tracking
KW - Human skeletal muscle cells
KW - Magnetic resonance imaging
KW - Superparamagnetic iron oxide
KW - Transplantation
UR - http://www.scopus.com/inward/record.url?scp=84861480825&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861480825&partnerID=8YFLogxK
U2 - 10.1007/s11307-011-0474-6
DO - 10.1007/s11307-011-0474-6
M3 - Article
C2 - 21336878
AN - SCOPUS:84861480825
VL - 14
SP - 47
EP - 59
JO - Molecular Imaging and Biology
JF - Molecular Imaging and Biology
SN - 1536-1632
IS - 1
ER -