Laboratory diagnosis of lupus anticoagulants: Effect of residual platelets in plasma, assessed by Staclot® LA and silica clotting time

Veena Chantarangkul, Armando Tripodi, Marigrazia Clerici, Caterina Bressi, Pier Mannuccio Mannucci

Research output: Contribution to journalArticlepeer-review

Abstract

Residual platelets in plasma are considered detrimental after freezing-thawing, as phospholipids released from ruptured platelets may quench lupus anticoagulants (LA). We aimed at assessing the effect of residual platelets after freezing-thawing plasmas tested with two procedures for LA. Blood from 52 patients suspected of having LA were centrifuged at 2,500 g. Plasmas were subdivided into 2 aliquots. One was filtered to remove residual platelets and both were frozen and stored at 70°C. Silica clotting time (SCT) at low and high phospholipid concentrations and Staclot® LA with and without Hexagonal phospholipids were performed on thawed plasmas. Plasmas were considered LA-positive when both SCT and Staclot® LA performed on filtered plasmas were diagnostic for LA. Forty-two of 52 plasmas fulfilled the diagnostic criteria and were retained for subsequent analysis. SCT on non-filtered plasmas was diagnostic for LA in 42 of 42 plasmas. Though the median (range) percentage correction recorded after phospholipids addition for filtered plasmas, i.e., 67% (36%-83%) was reduced to 54% (25%-81%) for non-filtered plasmas (p

Original languageEnglish
Pages (from-to)854-858
Number of pages5
JournalThrombosis and Haemostasis
Volume87
Issue number5
Publication statusPublished - 2002

Keywords

  • Lupus anticoagulant
  • Screening
  • Standardization
  • Thrombophilia

ASJC Scopus subject areas

  • Hematology

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