Laboratory diagnosis of lupus anticoagulants for patients on oral anticoagulant treatment: Performance of dilute Russell viper venom test and silica clotting time in comparison with Staclot® LA

Research output: Contribution to journalArticle

Abstract

The detection of lupus anticoagulant (LA) in plasmas from patients on oral anticoagulants is problematic because of their prolonged clotting times. Mixing of patients and normal plasmas prior to testing for LA is employed to overcome this problem. We investigated the diagnostic efficacy of silica clotting time (SCT) and dilute Russell viper venom test (dRVVT) performed at low and high phospholipid concentrations, to diagnose LA in patients on oral anticoagulants, in comparison with Staclot® LA (Stago) performed with and without hexagonal phospholipids and normal plasma. Case materials were 114 filtered plasmas from patients on oral anticoagulants with (n = 62) and without (n = 52) the antiphospholipid syndrome. Plasmas were considered LA-positive when Staclot® LA (taken as the "gold standard") was diagnostic for LA. Forty-four plasmas were positive with Staclot® LA. Forty and 39 of these were also positive with SCT and dRVVT (sensitivity relative to Staclot® LA was 91% and 89%, respectively). Seventy plasmas were negative with Staclot® LA. Three of these were positive with both SCT and dRVVT (specificity relative to Staclot® LA was 96%). Kappa values for measure of agreement were 0.87 and 0.85 (p

Original languageEnglish
Pages (from-to)583-586
Number of pages4
JournalThrombosis and Haemostasis
Volume88
Issue number4
Publication statusPublished - Oct 1 2002

Keywords

  • Lupus anticoagulant
  • Oral anticoagulation
  • Thrombosis

ASJC Scopus subject areas

  • Hematology

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