Laboratory evaluation of a commercial polyurethane filter for white cell reduction of red blood cells under conditions mimicking bedside use

P. Rebulla, N. Greppi, C. Smacchia, R. Mantovani, G. Sirchia

Research output: Contribution to journalArticle

Abstract

Purpose. Bedside filtration of red blood cells (RBC) can be very convenient in institutions where a large number of patients suffering from beta ihalassemia and other blood diseases are regularly transfused. Because concern has been expressed that bedside filtration, which may last longer and cause component temperatures higher than laboratory filtration, may be less effective than the latter, we evaluated in the laboratory the performance of a new commercial WBC reduction filter designed for bedside use under conditions mimicking a high-temperature environment with prolonged filtration times. Methods. We tested the polyurethane Imugard III-RC4B filter (Terumo, Tokyo, Japan) with red cells suspended in saline-adenine-glucose-mannitol. We prepared 34 red cell units with the buffy-coat (RBC) and 44 without the buffy-coat (RBC-BC) by high-speed (HS) centrifugation; in addition, we prepared 10 RBC units by low-speed (LS) centrifugation. After 1-2 day storage at 4°C, units were incubated for 1 hour at 27°C and filtered at 27°C through Imugard-III-RC4B. Filtration was done at slow flow in 60-95 min/unit. For comparative purposes, 10 RBC-BC units prepared by HS centrifugation were filtered at 4°C by gravity in approximately 10 min (high flow, laboratory conditions). Red cell recovery was determined by the ratio of total post/pre-filtration Hb. Post-filtration WBC were counted in a Nageotte chamber with the 3% paraformaldehyde method (Transfusion 1994;34:453). Results (median and range): Type of component and study conditions Hb% recovery WBC count (×103/unit) RBC, HS, filtration at 27°C. slow flow 87 (81-100) 13 (3-63) RBC-BC, HS, filtration at 27°C, slow flow 91 (85-97) 29 (6-2542) RBC, LS, filtration at 27°C, slow flow 90 (84-97) 50 (14-114) RBC-BC, HS, filtration at 4°C. high flow 87 (82-90) 6 (2-35) Conclusions. No filtered unit contained >2.6×106 WBC. Median values were ≤50,000 WBC/unit. Our data support the bedside use of this filter.

Original languageEnglish
Pages (from-to)284
Number of pages1
JournalInfusionstherapie und Transfusionsmedizin
Volume24
Issue number4
Publication statusPublished - 1997

Fingerprint

polyurethanes
Polyurethanes
erythrocytes
Erythrocytes
cells
leukocytes
Centrifugation
centrifugation
hematologic diseases
Temperature
Tokyo
Hematologic Diseases
Gravitation
Mannitol
Adenine
adenine
mannitol
leukocyte count
gravity
Japan

ASJC Scopus subject areas

  • Food Science
  • Immunology
  • Hematology

Cite this

Laboratory evaluation of a commercial polyurethane filter for white cell reduction of red blood cells under conditions mimicking bedside use. / Rebulla, P.; Greppi, N.; Smacchia, C.; Mantovani, R.; Sirchia, G.

In: Infusionstherapie und Transfusionsmedizin, Vol. 24, No. 4, 1997, p. 284.

Research output: Contribution to journalArticle

@article{e3e1ff0a90a44981901138a3be8822a6,
title = "Laboratory evaluation of a commercial polyurethane filter for white cell reduction of red blood cells under conditions mimicking bedside use",
abstract = "Purpose. Bedside filtration of red blood cells (RBC) can be very convenient in institutions where a large number of patients suffering from beta ihalassemia and other blood diseases are regularly transfused. Because concern has been expressed that bedside filtration, which may last longer and cause component temperatures higher than laboratory filtration, may be less effective than the latter, we evaluated in the laboratory the performance of a new commercial WBC reduction filter designed for bedside use under conditions mimicking a high-temperature environment with prolonged filtration times. Methods. We tested the polyurethane Imugard III-RC4B filter (Terumo, Tokyo, Japan) with red cells suspended in saline-adenine-glucose-mannitol. We prepared 34 red cell units with the buffy-coat (RBC) and 44 without the buffy-coat (RBC-BC) by high-speed (HS) centrifugation; in addition, we prepared 10 RBC units by low-speed (LS) centrifugation. After 1-2 day storage at 4°C, units were incubated for 1 hour at 27°C and filtered at 27°C through Imugard-III-RC4B. Filtration was done at slow flow in 60-95 min/unit. For comparative purposes, 10 RBC-BC units prepared by HS centrifugation were filtered at 4°C by gravity in approximately 10 min (high flow, laboratory conditions). Red cell recovery was determined by the ratio of total post/pre-filtration Hb. Post-filtration WBC were counted in a Nageotte chamber with the 3{\%} paraformaldehyde method (Transfusion 1994;34:453). Results (median and range): Type of component and study conditions Hb{\%} recovery WBC count (×103/unit) RBC, HS, filtration at 27°C. slow flow 87 (81-100) 13 (3-63) RBC-BC, HS, filtration at 27°C, slow flow 91 (85-97) 29 (6-2542) RBC, LS, filtration at 27°C, slow flow 90 (84-97) 50 (14-114) RBC-BC, HS, filtration at 4°C. high flow 87 (82-90) 6 (2-35) Conclusions. No filtered unit contained >2.6×106 WBC. Median values were ≤50,000 WBC/unit. Our data support the bedside use of this filter.",
author = "P. Rebulla and N. Greppi and C. Smacchia and R. Mantovani and G. Sirchia",
year = "1997",
language = "English",
volume = "24",
pages = "284",
journal = "Infusionstherapie und Transfusionsmedizin",
issn = "1424-5485",
publisher = "S. Karger AG",
number = "4",

}

TY - JOUR

T1 - Laboratory evaluation of a commercial polyurethane filter for white cell reduction of red blood cells under conditions mimicking bedside use

AU - Rebulla, P.

AU - Greppi, N.

AU - Smacchia, C.

AU - Mantovani, R.

AU - Sirchia, G.

PY - 1997

Y1 - 1997

N2 - Purpose. Bedside filtration of red blood cells (RBC) can be very convenient in institutions where a large number of patients suffering from beta ihalassemia and other blood diseases are regularly transfused. Because concern has been expressed that bedside filtration, which may last longer and cause component temperatures higher than laboratory filtration, may be less effective than the latter, we evaluated in the laboratory the performance of a new commercial WBC reduction filter designed for bedside use under conditions mimicking a high-temperature environment with prolonged filtration times. Methods. We tested the polyurethane Imugard III-RC4B filter (Terumo, Tokyo, Japan) with red cells suspended in saline-adenine-glucose-mannitol. We prepared 34 red cell units with the buffy-coat (RBC) and 44 without the buffy-coat (RBC-BC) by high-speed (HS) centrifugation; in addition, we prepared 10 RBC units by low-speed (LS) centrifugation. After 1-2 day storage at 4°C, units were incubated for 1 hour at 27°C and filtered at 27°C through Imugard-III-RC4B. Filtration was done at slow flow in 60-95 min/unit. For comparative purposes, 10 RBC-BC units prepared by HS centrifugation were filtered at 4°C by gravity in approximately 10 min (high flow, laboratory conditions). Red cell recovery was determined by the ratio of total post/pre-filtration Hb. Post-filtration WBC were counted in a Nageotte chamber with the 3% paraformaldehyde method (Transfusion 1994;34:453). Results (median and range): Type of component and study conditions Hb% recovery WBC count (×103/unit) RBC, HS, filtration at 27°C. slow flow 87 (81-100) 13 (3-63) RBC-BC, HS, filtration at 27°C, slow flow 91 (85-97) 29 (6-2542) RBC, LS, filtration at 27°C, slow flow 90 (84-97) 50 (14-114) RBC-BC, HS, filtration at 4°C. high flow 87 (82-90) 6 (2-35) Conclusions. No filtered unit contained >2.6×106 WBC. Median values were ≤50,000 WBC/unit. Our data support the bedside use of this filter.

AB - Purpose. Bedside filtration of red blood cells (RBC) can be very convenient in institutions where a large number of patients suffering from beta ihalassemia and other blood diseases are regularly transfused. Because concern has been expressed that bedside filtration, which may last longer and cause component temperatures higher than laboratory filtration, may be less effective than the latter, we evaluated in the laboratory the performance of a new commercial WBC reduction filter designed for bedside use under conditions mimicking a high-temperature environment with prolonged filtration times. Methods. We tested the polyurethane Imugard III-RC4B filter (Terumo, Tokyo, Japan) with red cells suspended in saline-adenine-glucose-mannitol. We prepared 34 red cell units with the buffy-coat (RBC) and 44 without the buffy-coat (RBC-BC) by high-speed (HS) centrifugation; in addition, we prepared 10 RBC units by low-speed (LS) centrifugation. After 1-2 day storage at 4°C, units were incubated for 1 hour at 27°C and filtered at 27°C through Imugard-III-RC4B. Filtration was done at slow flow in 60-95 min/unit. For comparative purposes, 10 RBC-BC units prepared by HS centrifugation were filtered at 4°C by gravity in approximately 10 min (high flow, laboratory conditions). Red cell recovery was determined by the ratio of total post/pre-filtration Hb. Post-filtration WBC were counted in a Nageotte chamber with the 3% paraformaldehyde method (Transfusion 1994;34:453). Results (median and range): Type of component and study conditions Hb% recovery WBC count (×103/unit) RBC, HS, filtration at 27°C. slow flow 87 (81-100) 13 (3-63) RBC-BC, HS, filtration at 27°C, slow flow 91 (85-97) 29 (6-2542) RBC, LS, filtration at 27°C, slow flow 90 (84-97) 50 (14-114) RBC-BC, HS, filtration at 4°C. high flow 87 (82-90) 6 (2-35) Conclusions. No filtered unit contained >2.6×106 WBC. Median values were ≤50,000 WBC/unit. Our data support the bedside use of this filter.

UR - http://www.scopus.com/inward/record.url?scp=33751244575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751244575&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33751244575

VL - 24

SP - 284

JO - Infusionstherapie und Transfusionsmedizin

JF - Infusionstherapie und Transfusionsmedizin

SN - 1424-5485

IS - 4

ER -