Lack of a correlation between p53 protein expression and radiation response in human tumor primary cultures

N. Zaffaroni, E. Benini, D. Gornati, A. Bearzatto, R. Silvestrini

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the possible relationship between immunohistochemically detected p53 expression and in vitro response to γ-irradiation in 24 primary cultures of human ovarian cancers and cutaneous melanomas. The frequency of p53-positive tumors was around 60% within each tumor histotype. The range of the surviving fraction at 2 Gy (SF2) was similar in p53-positive (0.10- 0.76) and p53-negative (0.23-0.65) tumors, with median values of 0.36 and 0.33, respectively. No differences were observed in the accumulation of DNA- double strand breaks, assessed by neutral filter elution after exposure to 50 Gy, between p53-positive and p53-negative tumors. As regards DNA lesion repair, after 2 h of recovery the percentage of rejoined DNA-double strand breaks ranged from 19% to 99% in the different cultures, but again the distribution of values was similar for p53-positive and p53-negative tumors. Specifically, the median percentage of repaired DNA-double strand breaks was 70% and 74% in the two groups. On the whole, our data do not support the hypothesis that p53 overexpression is a major determinant of in vitro radiation response.

Original languageEnglish
Pages (from-to)77-85
Number of pages9
JournalStem Cells
Volume13
Issue number1
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Cell Biology

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