Lack of activation of peripheral blood dendritic cells in human pregnancies complicated by intrauterine growth restriction

M. Cappelletti, S. Giannelli, A. Martinelli, I. Cetin, E. Colombo, F. Calcaterra, D. Mavilio, S. Della Bella

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Introduction: The state of activation of dendritic cells (DCs) at the feto-maternal interface critically contributes to optimal decidual immune responses needed to support fetal-placental development. We recently demonstrated that during healthy pregnancy also peripheral blood DCs (PBDCs), which are easily accessible, are activated as well. In this study, to investigate a possible involvement of DCs in intrauterine growth restriction (IUGR), we evaluated whether PBDCs in pregnancy complicated by IUGR may be altered compared with PBDCs in healthy pregnancy. Methods: PBDCs from 12 pregnant women with primary IUGR, 21 healthy pregnant and 19 nonpregnant women were analyzed by flow cytometric analysis of whole-blood samples collected at a single time point. Results: The number of plasmacytoid PBDCs was significantly reduced in women with IUGR pregnancy. Myeloid and plasmacytoid PBDCs in IUGR lacked the state of activation (assessed as CD80, CD86, CD40 expression) and the shift to a proinflammatory pattern of cytokine production occurring during healthy pregnancy. Discussion: To our knowledge, this is the first study investigating the state of PBDC activation in IUGR pregnancy. Our results are in accordance with a previous study reporting a lower expression of activation and maturation markers by decidual DCs in IUGR placentas. Conclusions: The reduced activation of PBDCs in IUGR pregnancy may possibly reflect a reduced activation of decidual DCs. If confirmed at the feto-maternal interface, the alterations of DCs described in IUGR pregnancy have the potential to negatively impact on vascular development during gestation. These observations may therefore broaden our understanding of IUGR pathogenesis.

Original languageEnglish
Pages (from-to)35-41
Number of pages7
JournalPlacenta
Volume34
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Dendritic Cells
Blood Cells
Pregnancy
Growth
Mothers
Placentation
Fetal Development
Placenta
Blood Vessels
Pregnant Women
Cytokines

Keywords

  • Costimulatory molecules
  • Cytokines
  • Intrauterine growth restriction
  • Peripheral blood dendritic cells

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Developmental Biology

Cite this

Lack of activation of peripheral blood dendritic cells in human pregnancies complicated by intrauterine growth restriction. / Cappelletti, M.; Giannelli, S.; Martinelli, A.; Cetin, I.; Colombo, E.; Calcaterra, F.; Mavilio, D.; Della Bella, S.

In: Placenta, Vol. 34, No. 1, 01.2013, p. 35-41.

Research output: Contribution to journalArticle

Cappelletti, M. ; Giannelli, S. ; Martinelli, A. ; Cetin, I. ; Colombo, E. ; Calcaterra, F. ; Mavilio, D. ; Della Bella, S. / Lack of activation of peripheral blood dendritic cells in human pregnancies complicated by intrauterine growth restriction. In: Placenta. 2013 ; Vol. 34, No. 1. pp. 35-41.
@article{b0c10fd5592145b2ad00cbe8c0196188,
title = "Lack of activation of peripheral blood dendritic cells in human pregnancies complicated by intrauterine growth restriction",
abstract = "Introduction: The state of activation of dendritic cells (DCs) at the feto-maternal interface critically contributes to optimal decidual immune responses needed to support fetal-placental development. We recently demonstrated that during healthy pregnancy also peripheral blood DCs (PBDCs), which are easily accessible, are activated as well. In this study, to investigate a possible involvement of DCs in intrauterine growth restriction (IUGR), we evaluated whether PBDCs in pregnancy complicated by IUGR may be altered compared with PBDCs in healthy pregnancy. Methods: PBDCs from 12 pregnant women with primary IUGR, 21 healthy pregnant and 19 nonpregnant women were analyzed by flow cytometric analysis of whole-blood samples collected at a single time point. Results: The number of plasmacytoid PBDCs was significantly reduced in women with IUGR pregnancy. Myeloid and plasmacytoid PBDCs in IUGR lacked the state of activation (assessed as CD80, CD86, CD40 expression) and the shift to a proinflammatory pattern of cytokine production occurring during healthy pregnancy. Discussion: To our knowledge, this is the first study investigating the state of PBDC activation in IUGR pregnancy. Our results are in accordance with a previous study reporting a lower expression of activation and maturation markers by decidual DCs in IUGR placentas. Conclusions: The reduced activation of PBDCs in IUGR pregnancy may possibly reflect a reduced activation of decidual DCs. If confirmed at the feto-maternal interface, the alterations of DCs described in IUGR pregnancy have the potential to negatively impact on vascular development during gestation. These observations may therefore broaden our understanding of IUGR pathogenesis.",
keywords = "Costimulatory molecules, Cytokines, Intrauterine growth restriction, Peripheral blood dendritic cells",
author = "M. Cappelletti and S. Giannelli and A. Martinelli and I. Cetin and E. Colombo and F. Calcaterra and D. Mavilio and {Della Bella}, S.",
year = "2013",
month = "1",
doi = "10.1016/j.placenta.2012.10.016",
language = "English",
volume = "34",
pages = "35--41",
journal = "Placenta",
issn = "0143-4004",
publisher = "W.B. Saunders Ltd",
number = "1",

}

TY - JOUR

T1 - Lack of activation of peripheral blood dendritic cells in human pregnancies complicated by intrauterine growth restriction

AU - Cappelletti, M.

AU - Giannelli, S.

AU - Martinelli, A.

AU - Cetin, I.

AU - Colombo, E.

AU - Calcaterra, F.

AU - Mavilio, D.

AU - Della Bella, S.

PY - 2013/1

Y1 - 2013/1

N2 - Introduction: The state of activation of dendritic cells (DCs) at the feto-maternal interface critically contributes to optimal decidual immune responses needed to support fetal-placental development. We recently demonstrated that during healthy pregnancy also peripheral blood DCs (PBDCs), which are easily accessible, are activated as well. In this study, to investigate a possible involvement of DCs in intrauterine growth restriction (IUGR), we evaluated whether PBDCs in pregnancy complicated by IUGR may be altered compared with PBDCs in healthy pregnancy. Methods: PBDCs from 12 pregnant women with primary IUGR, 21 healthy pregnant and 19 nonpregnant women were analyzed by flow cytometric analysis of whole-blood samples collected at a single time point. Results: The number of plasmacytoid PBDCs was significantly reduced in women with IUGR pregnancy. Myeloid and plasmacytoid PBDCs in IUGR lacked the state of activation (assessed as CD80, CD86, CD40 expression) and the shift to a proinflammatory pattern of cytokine production occurring during healthy pregnancy. Discussion: To our knowledge, this is the first study investigating the state of PBDC activation in IUGR pregnancy. Our results are in accordance with a previous study reporting a lower expression of activation and maturation markers by decidual DCs in IUGR placentas. Conclusions: The reduced activation of PBDCs in IUGR pregnancy may possibly reflect a reduced activation of decidual DCs. If confirmed at the feto-maternal interface, the alterations of DCs described in IUGR pregnancy have the potential to negatively impact on vascular development during gestation. These observations may therefore broaden our understanding of IUGR pathogenesis.

AB - Introduction: The state of activation of dendritic cells (DCs) at the feto-maternal interface critically contributes to optimal decidual immune responses needed to support fetal-placental development. We recently demonstrated that during healthy pregnancy also peripheral blood DCs (PBDCs), which are easily accessible, are activated as well. In this study, to investigate a possible involvement of DCs in intrauterine growth restriction (IUGR), we evaluated whether PBDCs in pregnancy complicated by IUGR may be altered compared with PBDCs in healthy pregnancy. Methods: PBDCs from 12 pregnant women with primary IUGR, 21 healthy pregnant and 19 nonpregnant women were analyzed by flow cytometric analysis of whole-blood samples collected at a single time point. Results: The number of plasmacytoid PBDCs was significantly reduced in women with IUGR pregnancy. Myeloid and plasmacytoid PBDCs in IUGR lacked the state of activation (assessed as CD80, CD86, CD40 expression) and the shift to a proinflammatory pattern of cytokine production occurring during healthy pregnancy. Discussion: To our knowledge, this is the first study investigating the state of PBDC activation in IUGR pregnancy. Our results are in accordance with a previous study reporting a lower expression of activation and maturation markers by decidual DCs in IUGR placentas. Conclusions: The reduced activation of PBDCs in IUGR pregnancy may possibly reflect a reduced activation of decidual DCs. If confirmed at the feto-maternal interface, the alterations of DCs described in IUGR pregnancy have the potential to negatively impact on vascular development during gestation. These observations may therefore broaden our understanding of IUGR pathogenesis.

KW - Costimulatory molecules

KW - Cytokines

KW - Intrauterine growth restriction

KW - Peripheral blood dendritic cells

UR - http://www.scopus.com/inward/record.url?scp=84872050144&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872050144&partnerID=8YFLogxK

U2 - 10.1016/j.placenta.2012.10.016

DO - 10.1016/j.placenta.2012.10.016

M3 - Article

C2 - 23182380

AN - SCOPUS:84872050144

VL - 34

SP - 35

EP - 41

JO - Placenta

JF - Placenta

SN - 0143-4004

IS - 1

ER -