Lack of association between carotid intima-media thickness and methylenetetrahydrofolate reductase gene polymorphism or serum homocysteine in non-insulin-dependent diabetes mellitus

A. Mazza, C. Motti, A. Nulli, G. Marra, A. Gnasso, A. Pastore, G. Federici, C. Cortese

Research output: Contribution to journalArticle

Abstract

We assessed the contribution of the serum homocysteine (Hcy) level, an independent risk factor for vascular disease, and methylene tetrahydrofolate reductase (MTHFR) gene polymorphism to the variability of intimal-medial thickness (IMT) of the common carotid artery in middle-aged non-insulin- dependent diabetes mellitus (NIDDM) subjects. One hundred thirty NIDDM patients (60 males and 70 females) with a mean age of 53 ± 10 years and a mean diabetes duration of 11.3 ± 7.9 years were enrolled for the study. Exclusion criteria included liver, heart, kidney, or other major-organ disease. Fasting total serum Hcy, folate, and vitamin B12 and clinical chemistry analyte levels were measured. MTHFR polymorphism was determined by polymerase chain reaction (PCR). IMT and plaques or stenosis in the common carotid were measured by ultrasonography. Serum Hcy was inversely correlated with vitamin levels and was slightly higher in subjects with the Val/Val genotype versus Ala/Val and Ala/Ala (P = .02); no differences in genotype were found in subjects with folate or vitamin B12 at or above the median level. In univariate analysis, common carotid IMT was significantly associated with age (P = .00001), the body mass index ([BMI] P = .0003), uric acid (P = .004), systolic blood pressure (P = .03), glycemia (P = .03), and total cholesterol (P = .04). No significant association was found between serum Hcy or MTHFR polymorphism and IMT. In multiple regression analysis, age (P = .0001), uric acid (P = .03), glycemia, and the BMI (P = .05) were independently associated with IMT and explained about 42% of IMT variability. In 130 NIDDM patients without nephropathy, basal levels of serum Hcy, as well as MTHFR polymorphism, did not predict significant changes in common carotid IMT. Copyright (C) 2000 by W.B. Saunders Company.

Original languageEnglish
Pages (from-to)718-723
Number of pages6
JournalMetabolism
Volume49
Issue number6
Publication statusPublished - 2000

Fingerprint

Tunica Intima
Methylenetetrahydrofolate Reductase (NADPH2)
Carotid Intima-Media Thickness
Homocysteine
Type 2 Diabetes Mellitus
Serum
Genes
Vitamin B 12
Uric Acid
Folic Acid
Body Mass Index
Genotype
Blood Pressure
Clinical Chemistry
Common Carotid Artery
Vascular Diseases
Vitamins
Fasting
Ultrasonography
Pathologic Constriction

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Lack of association between carotid intima-media thickness and methylenetetrahydrofolate reductase gene polymorphism or serum homocysteine in non-insulin-dependent diabetes mellitus. / Mazza, A.; Motti, C.; Nulli, A.; Marra, G.; Gnasso, A.; Pastore, A.; Federici, G.; Cortese, C.

In: Metabolism, Vol. 49, No. 6, 2000, p. 718-723.

Research output: Contribution to journalArticle

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AU - Motti, C.

AU - Nulli, A.

AU - Marra, G.

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