Lack of association between UGT1A7, UGT1A9, ARP, SPINK1 and CFTR gene polymorphisms and pancreatic cancer in Italian patients

Ada Piepoli, Annamaria Gentile, Maria Rosa Valvano, Daniela Barana, Cristina Oliani, Rosa Cotugno, Michele Quitadamo, Angelo Andriuli, Francesco Perri

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: To investigate simultaneously UGT1A7, UGT1A9, ARP, SPINK and CFTR genes to verify whether genetic polymorphisms predispose to the development of pancreatic cancer (PC). Methods: Genomic DNA of 61 pancreatic cancer patients and 105 healthy controls (HC) were analyzed. UGT1A7 genotyping was determined by PCR-RFLP analysis. Specific PCR and sequencing were used to analyze genetic variants of UGT1A9, ARP, SPINK1 and CFTR genes. Results: Four different alieles (*1: WT; *2: N129K and R131K; *3: N129K, R131K, and W20811; and *4: W208R) in UGT1A7 and three different alleles (*1: WT; *4: Y242X; and *5: D256N) in UGT1A9 were detected. All UGT1A polymorphisms were observed at similar frequency in PC patients and HC. Seven different alleles in ARP were found in PC patients and HC at similar frequency. The SPINK1 mutations N34S and P55S occurred in five PC patients with a prevalence (4.1%) not significantly different from that observed (2.0%) in HC. The only CFTR ΔF508 mutation was recognized in three PC patients with a prevalence (4.9%) similar to HC. Conclusion: UGT1A7, UGT1A9, ARP, SPINK1 and CFTR gene polymorphisms are not associated with PC in Italian patients.

Original languageEnglish
Pages (from-to)6343-6348
Number of pages6
JournalWorld Journal of Gastroenterology
Volume12
Issue number39
Publication statusPublished - Oct 21 2006

Keywords

  • Genetic polymorphisms
  • Pancreatic cancer
  • Risk factors
  • Sporadic

ASJC Scopus subject areas

  • Gastroenterology

Fingerprint Dive into the research topics of 'Lack of association between UGT1A7, UGT1A9, ARP, SPINK1 and CFTR gene polymorphisms and pancreatic cancer in Italian patients'. Together they form a unique fingerprint.

Cite this