Lack of Bcl10 gene mutations in laryngeal squamous cell carcinoma

Domenica Ronchetti, Daniela Intini, Giancarlo Pruneri, Antonino Neri, Lorenzo Pignataro

Research output: Contribution to journalArticlepeer-review

Abstract

The Bcl10 gene encodes a protein probably involved in some apoptotic regulatory pathways. Bcl10 mutations lead to the translation of truncated proteins that show gain-of-function transforming activity; it has been suggested that Bcl10 may represent a major target gene for inactivation in many human cancers. To define the frequency of Bcl10 mutations in laryngeal squamous cell carcinoma and their possible association with tumour progression, we investigated a large panel of tumours representative of all grades and stages of malignancy. To detect pathogenic mutations in exons 1, 2 and 3 of the Bcl10 gene, we performed a silver-staining polymerase chain reaction - single-strand conformation polymorphism (PCR-SSCP) analysis followed by direct DNA sequencing. We revealed the presence of SSCP variants in 18 out of 91 laryngeal tumours. Direct DNA sequencing showed previously described polymorphisms but no pathogenic mutations. We have strong evidence that the Bcl10 gene is not involved in laryngeal carcinogenesis.

Original languageEnglish
Pages (from-to)610-612
Number of pages3
JournalJournal of Laryngology and Otology
Volume116
Issue number8
DOIs
Publication statusPublished - 2002

Keywords

  • Apoptosis
  • Carcinoma
  • Genes
  • Larynx
  • Squamous Cell

ASJC Scopus subject areas

  • Otorhinolaryngology

Fingerprint Dive into the research topics of 'Lack of Bcl10 gene mutations in laryngeal squamous cell carcinoma'. Together they form a unique fingerprint.

Cite this