Lack of BRAF mutation in primary uveal melanoma

Yoram Cohen, Nitza Goldenberg-Cohen, Paola Parrella, Itay Chowers, Shannath L. Merbs, Jacob Pe'er, David Sidransky

Research output: Contribution to journalArticle

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Abstract

PURPOSE. BRAF T1796A activating mutations have been found in a high proportion of cutaneous melanomas, cutaneous nevi, and papillary thyroid carcinoma and in a small fraction of other cancers. This study was designed to investigate the incidence of BRAF T1796A mutation in uveal melanoma. METHODS. Twenty-nine formalin-fixed, paraffin-embedded posterior uveal melanomas were included in the study. DNA was extracted from the paraffin sections followed by PCR amplification of exon 15 and detection of the common BRAF missense mutation (T→A transversion at nucleotide 1796) using restriction enzyme analysis. RESULTS. Although positive cutaneous melanoma control cell lines harbored the T1796A BRAF mutation, none of the 29 uveal melanomas harbored the mutation. CONCLUSIONS. These data suggest that BRAF T1796A activating mutation is not common in primary uveal melanoma. These findings are in accord with known differences in tumorigenesis between uveal and cutaneous melanomas.

Original languageEnglish
Pages (from-to)2876-2878
Number of pages3
JournalInvestigative Ophthalmology and Visual Science
Volume44
Issue number7
DOIs
Publication statusPublished - Jul 1 2003

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Mutation
Skin
Paraffin
Nevi and Melanomas
Restriction Mapping
Missense Mutation
Formaldehyde
Exons
Melanoma
Carcinogenesis
Nucleotides
Uveal melanoma
Cell Line
Polymerase Chain Reaction
DNA
Incidence
Neoplasms

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Cohen, Y., Goldenberg-Cohen, N., Parrella, P., Chowers, I., Merbs, S. L., Pe'er, J., & Sidransky, D. (2003). Lack of BRAF mutation in primary uveal melanoma. Investigative Ophthalmology and Visual Science, 44(7), 2876-2878. https://doi.org/10.1167/iovs.02-1329

Lack of BRAF mutation in primary uveal melanoma. / Cohen, Yoram; Goldenberg-Cohen, Nitza; Parrella, Paola; Chowers, Itay; Merbs, Shannath L.; Pe'er, Jacob; Sidransky, David.

In: Investigative Ophthalmology and Visual Science, Vol. 44, No. 7, 01.07.2003, p. 2876-2878.

Research output: Contribution to journalArticle

Cohen, Y, Goldenberg-Cohen, N, Parrella, P, Chowers, I, Merbs, SL, Pe'er, J & Sidransky, D 2003, 'Lack of BRAF mutation in primary uveal melanoma', Investigative Ophthalmology and Visual Science, vol. 44, no. 7, pp. 2876-2878. https://doi.org/10.1167/iovs.02-1329
Cohen Y, Goldenberg-Cohen N, Parrella P, Chowers I, Merbs SL, Pe'er J et al. Lack of BRAF mutation in primary uveal melanoma. Investigative Ophthalmology and Visual Science. 2003 Jul 1;44(7):2876-2878. https://doi.org/10.1167/iovs.02-1329
Cohen, Yoram ; Goldenberg-Cohen, Nitza ; Parrella, Paola ; Chowers, Itay ; Merbs, Shannath L. ; Pe'er, Jacob ; Sidransky, David. / Lack of BRAF mutation in primary uveal melanoma. In: Investigative Ophthalmology and Visual Science. 2003 ; Vol. 44, No. 7. pp. 2876-2878.
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AB - PURPOSE. BRAF T1796A activating mutations have been found in a high proportion of cutaneous melanomas, cutaneous nevi, and papillary thyroid carcinoma and in a small fraction of other cancers. This study was designed to investigate the incidence of BRAF T1796A mutation in uveal melanoma. METHODS. Twenty-nine formalin-fixed, paraffin-embedded posterior uveal melanomas were included in the study. DNA was extracted from the paraffin sections followed by PCR amplification of exon 15 and detection of the common BRAF missense mutation (T→A transversion at nucleotide 1796) using restriction enzyme analysis. RESULTS. Although positive cutaneous melanoma control cell lines harbored the T1796A BRAF mutation, none of the 29 uveal melanomas harbored the mutation. CONCLUSIONS. These data suggest that BRAF T1796A activating mutation is not common in primary uveal melanoma. These findings are in accord with known differences in tumorigenesis between uveal and cutaneous melanomas.

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