Lack of compensatory megakaryocytopoiesis in HIV-1-seropositive thrombocytopenic individuals compared with immune thrombocytopenic purpura patients

Giorgio Zauli, Maria Carla Re, Luigi Gugliotta, Giuseppe Visani, Nicola Vianelli, Giuliano Furlini, Michele La Placa

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Abstract

In this study we demonstrate that HIV-1-seropositive thrombocytopenic individuals, in contrast with immune thrombocytopenic purpura (ITP) patients, fail to have a compensatory increase of megakaryocytopoiesis. The in vitro growth of bone-marrow megakaryocyte progenitors (CFU-MK) and the production of granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-1 and IL-6 by bone-marrow mononuclear adherent cells and peripheral blood (PB) light-density mononuclear cells were studied in 12 HIV-1-seropositive thromobocytopenic individuals with respect to 12 ITP patients and 15 normal controls. In HIV-1-seropositive thrombocytopenic individuals, CFU-MK size (number of megakaryocytes per colony) was similar to normal controls but significantly lower (P <0.05) than in ITP patients. IL-1 and IL-6 production was similar in the three groups of subjects. On the other hand, GM-CSF production by bone-marrow mononuclear adherent cells in HIV-1-seropositive thrombocytopenic individuals was similar to normal controls but significantly (P <0.05) lower than in ITP patients, whereas GM-CSF production by PB light-density mononuclear cells was markedly (P <0.05) defective compared with both normal controls and ITP patients. The positive correlation between number and size of CFU-MK and production of GM-CSF by bone-marrow mononuclear adherent cells, observed in all three groups of subjects, demonstrates the central role of GM-CSF in the control of megakaryocytopoiesis.

Original languageEnglish
Pages (from-to)1345-1350
Number of pages6
JournalAIDS (London, England)
Volume5
Issue number11
Publication statusPublished - Nov 1991

Fingerprint

Thrombopoiesis
Idiopathic Thrombocytopenic Purpura
Granulocyte-Macrophage Colony-Stimulating Factor
HIV-1
Bone Marrow
Interleukin-1
Interleukin-6
Cell Count
Megakaryocyte Progenitor Cells
Light
Macrophage Colony-Stimulating Factor
Megakaryocytes
Blood Cells
Growth

Keywords

  • HIV
  • Interleukin 1 and 6
  • Megakaryocyte progenitors granulocyte/macrophage colony-stimulating factor
  • Thrombocytopenia

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Zauli, G., Re, M. C., Gugliotta, L., Visani, G., Vianelli, N., Furlini, G., & La Placa, M. (1991). Lack of compensatory megakaryocytopoiesis in HIV-1-seropositive thrombocytopenic individuals compared with immune thrombocytopenic purpura patients. AIDS (London, England), 5(11), 1345-1350.

Lack of compensatory megakaryocytopoiesis in HIV-1-seropositive thrombocytopenic individuals compared with immune thrombocytopenic purpura patients. / Zauli, Giorgio; Re, Maria Carla; Gugliotta, Luigi; Visani, Giuseppe; Vianelli, Nicola; Furlini, Giuliano; La Placa, Michele.

In: AIDS (London, England), Vol. 5, No. 11, 11.1991, p. 1345-1350.

Research output: Contribution to journalArticle

Zauli, G, Re, MC, Gugliotta, L, Visani, G, Vianelli, N, Furlini, G & La Placa, M 1991, 'Lack of compensatory megakaryocytopoiesis in HIV-1-seropositive thrombocytopenic individuals compared with immune thrombocytopenic purpura patients', AIDS (London, England), vol. 5, no. 11, pp. 1345-1350.
Zauli, Giorgio ; Re, Maria Carla ; Gugliotta, Luigi ; Visani, Giuseppe ; Vianelli, Nicola ; Furlini, Giuliano ; La Placa, Michele. / Lack of compensatory megakaryocytopoiesis in HIV-1-seropositive thrombocytopenic individuals compared with immune thrombocytopenic purpura patients. In: AIDS (London, England). 1991 ; Vol. 5, No. 11. pp. 1345-1350.
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