Abstract
In this study we demonstrate that HIV-1-seropositive thrombocytopenic individuals, in contrast with immune thrombocytopenic purpura (ITP) patients, fail to have a compensatory increase of megakaryocytopoiesis. The in vitro growth of bone-marrow megakaryocyte progenitors (CFU-MK) and the production of granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-1 and IL-6 by bone-marrow mononuclear adherent cells and peripheral blood (PB) light-density mononuclear cells were studied in 12 HIV-1-seropositive thromobocytopenic individuals with respect to 12 ITP patients and 15 normal controls. In HIV-1-seropositive thrombocytopenic individuals, CFU-MK size (number of megakaryocytes per colony) was similar to normal controls but significantly lower (P <0.05) than in ITP patients. IL-1 and IL-6 production was similar in the three groups of subjects. On the other hand, GM-CSF production by bone-marrow mononuclear adherent cells in HIV-1-seropositive thrombocytopenic individuals was similar to normal controls but significantly (P <0.05) lower than in ITP patients, whereas GM-CSF production by PB light-density mononuclear cells was markedly (P <0.05) defective compared with both normal controls and ITP patients. The positive correlation between number and size of CFU-MK and production of GM-CSF by bone-marrow mononuclear adherent cells, observed in all three groups of subjects, demonstrates the central role of GM-CSF in the control of megakaryocytopoiesis.
| Original language | English |
|---|---|
| Pages (from-to) | 1345-1350 |
| Number of pages | 6 |
| Journal | AIDS (London, England) |
| Volume | 5 |
| Issue number | 11 |
| Publication status | Published - Nov 1991 |
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Keywords
- HIV
- Interleukin 1 and 6
- Megakaryocyte progenitors granulocyte/macrophage colony-stimulating factor
- Thrombocytopenia
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Lack of compensatory megakaryocytopoiesis in HIV-1-seropositive thrombocytopenic individuals compared with immune thrombocytopenic purpura patients. / Zauli, Giorgio; Re, Maria Carla; Gugliotta, Luigi; Visani, Giuseppe; Vianelli, Nicola; Furlini, Giuliano; La Placa, Michele.
In: AIDS (London, England), Vol. 5, No. 11, 11.1991, p. 1345-1350.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lack of compensatory megakaryocytopoiesis in HIV-1-seropositive thrombocytopenic individuals compared with immune thrombocytopenic purpura patients
AU - Zauli, Giorgio
AU - Re, Maria Carla
AU - Gugliotta, Luigi
AU - Visani, Giuseppe
AU - Vianelli, Nicola
AU - Furlini, Giuliano
AU - La Placa, Michele
PY - 1991/11
Y1 - 1991/11
N2 - In this study we demonstrate that HIV-1-seropositive thrombocytopenic individuals, in contrast with immune thrombocytopenic purpura (ITP) patients, fail to have a compensatory increase of megakaryocytopoiesis. The in vitro growth of bone-marrow megakaryocyte progenitors (CFU-MK) and the production of granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-1 and IL-6 by bone-marrow mononuclear adherent cells and peripheral blood (PB) light-density mononuclear cells were studied in 12 HIV-1-seropositive thromobocytopenic individuals with respect to 12 ITP patients and 15 normal controls. In HIV-1-seropositive thrombocytopenic individuals, CFU-MK size (number of megakaryocytes per colony) was similar to normal controls but significantly lower (P <0.05) than in ITP patients. IL-1 and IL-6 production was similar in the three groups of subjects. On the other hand, GM-CSF production by bone-marrow mononuclear adherent cells in HIV-1-seropositive thrombocytopenic individuals was similar to normal controls but significantly (P <0.05) lower than in ITP patients, whereas GM-CSF production by PB light-density mononuclear cells was markedly (P <0.05) defective compared with both normal controls and ITP patients. The positive correlation between number and size of CFU-MK and production of GM-CSF by bone-marrow mononuclear adherent cells, observed in all three groups of subjects, demonstrates the central role of GM-CSF in the control of megakaryocytopoiesis.
AB - In this study we demonstrate that HIV-1-seropositive thrombocytopenic individuals, in contrast with immune thrombocytopenic purpura (ITP) patients, fail to have a compensatory increase of megakaryocytopoiesis. The in vitro growth of bone-marrow megakaryocyte progenitors (CFU-MK) and the production of granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-1 and IL-6 by bone-marrow mononuclear adherent cells and peripheral blood (PB) light-density mononuclear cells were studied in 12 HIV-1-seropositive thromobocytopenic individuals with respect to 12 ITP patients and 15 normal controls. In HIV-1-seropositive thrombocytopenic individuals, CFU-MK size (number of megakaryocytes per colony) was similar to normal controls but significantly lower (P <0.05) than in ITP patients. IL-1 and IL-6 production was similar in the three groups of subjects. On the other hand, GM-CSF production by bone-marrow mononuclear adherent cells in HIV-1-seropositive thrombocytopenic individuals was similar to normal controls but significantly (P <0.05) lower than in ITP patients, whereas GM-CSF production by PB light-density mononuclear cells was markedly (P <0.05) defective compared with both normal controls and ITP patients. The positive correlation between number and size of CFU-MK and production of GM-CSF by bone-marrow mononuclear adherent cells, observed in all three groups of subjects, demonstrates the central role of GM-CSF in the control of megakaryocytopoiesis.
KW - HIV
KW - Interleukin 1 and 6
KW - Megakaryocyte progenitors granulocyte/macrophage colony-stimulating factor
KW - Thrombocytopenia
UR - http://www.scopus.com/inward/record.url?scp=0026048650&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026048650&partnerID=8YFLogxK
M3 - Article
C2 - 1768383
AN - SCOPUS:0026048650
VL - 5
SP - 1345
EP - 1350
JO - AIDS
JF - AIDS
SN - 0269-9370
IS - 11
ER -