Abstract
In this study we demonstrate that HIV-1-seropositive thrombocytopenic individuals, in contrast with immune thrombocytopenic purpura (ITP) patients, fail to have a compensatory increase of megakaryocytopoiesis. The in vitro growth of bone-marrow megakaryocyte progenitors (CFU-MK) and the production of granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-1 and IL-6 by bone-marrow mononuclear adherent cells and peripheral blood (PB) light-density mononuclear cells were studied in 12 HIV-1-seropositive thromobocytopenic individuals with respect to 12 ITP patients and 15 normal controls. In HIV-1-seropositive thrombocytopenic individuals, CFU-MK size (number of megakaryocytes per colony) was similar to normal controls but significantly lower (P <0.05) than in ITP patients. IL-1 and IL-6 production was similar in the three groups of subjects. On the other hand, GM-CSF production by bone-marrow mononuclear adherent cells in HIV-1-seropositive thrombocytopenic individuals was similar to normal controls but significantly (P <0.05) lower than in ITP patients, whereas GM-CSF production by PB light-density mononuclear cells was markedly (P <0.05) defective compared with both normal controls and ITP patients. The positive correlation between number and size of CFU-MK and production of GM-CSF by bone-marrow mononuclear adherent cells, observed in all three groups of subjects, demonstrates the central role of GM-CSF in the control of megakaryocytopoiesis.
Original language | English |
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Pages (from-to) | 1345-1350 |
Number of pages | 6 |
Journal | AIDS (London, England) |
Volume | 5 |
Issue number | 11 |
Publication status | Published - Nov 1991 |
Keywords
- HIV
- Interleukin 1 and 6
- Megakaryocyte progenitors granulocyte/macrophage colony-stimulating factor
- Thrombocytopenia
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy