Lack of correlation between hypomethylation and expression of the HLA-DRα gene

R. Gambari, L. Del Senno, R. Barbieri, D. Buzzoni, K. Gustafsson, P. Giacomini, P. G. Natali

Research output: Contribution to journalArticle

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Abstract

DNA methylation at the 5'-CCGG-3' sites of the HLA-DRα gene and relative flanking regions has been analyzed by Msp I and Hpa II enzymatic digestion in order to determine whether a correlation exists between DNA methylation and transcription of the HLA-DRα gene. Unexpectedly, and in contrast to the behavior of most eukaryotic genes, no positive correlation was found between hypomethylation and expression. In fact, HLA-DRα appears to be fully unmethylated at MspI/Hpa II sites in K562 cells, not expressing DR molecules, and to exhibit a high degree of methylation in Colo 38 cells, which actively transcribe the gene. The unorthodox behavior of HLA-DRα is not unique to melanoma or erythroid cells since a similar, positive correlation between methylation and expression also exists when this analysis is extended to cell lines belonging to other histotypes.

Original languageEnglish
Pages (from-to)365-369
Number of pages5
JournalEuropean Journal of Immunology
Volume16
Issue number4
Publication statusPublished - 1986

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HLA-DR Antigens
DNA Methylation
Methylation
Genes
Erythroid Cells
K562 Cells
Digestion
Melanoma
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Gambari, R., Del Senno, L., Barbieri, R., Buzzoni, D., Gustafsson, K., Giacomini, P., & Natali, P. G. (1986). Lack of correlation between hypomethylation and expression of the HLA-DRα gene. European Journal of Immunology, 16(4), 365-369.

Lack of correlation between hypomethylation and expression of the HLA-DRα gene. / Gambari, R.; Del Senno, L.; Barbieri, R.; Buzzoni, D.; Gustafsson, K.; Giacomini, P.; Natali, P. G.

In: European Journal of Immunology, Vol. 16, No. 4, 1986, p. 365-369.

Research output: Contribution to journalArticle

Gambari, R, Del Senno, L, Barbieri, R, Buzzoni, D, Gustafsson, K, Giacomini, P & Natali, PG 1986, 'Lack of correlation between hypomethylation and expression of the HLA-DRα gene', European Journal of Immunology, vol. 16, no. 4, pp. 365-369.
Gambari R, Del Senno L, Barbieri R, Buzzoni D, Gustafsson K, Giacomini P et al. Lack of correlation between hypomethylation and expression of the HLA-DRα gene. European Journal of Immunology. 1986;16(4):365-369.
Gambari, R. ; Del Senno, L. ; Barbieri, R. ; Buzzoni, D. ; Gustafsson, K. ; Giacomini, P. ; Natali, P. G. / Lack of correlation between hypomethylation and expression of the HLA-DRα gene. In: European Journal of Immunology. 1986 ; Vol. 16, No. 4. pp. 365-369.
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