Lack of correlation between hypomethylation and expression of the HLA-DRα gene

R. Gambari, L. Del Senno, R. Barbieri, D. Buzzoni, K. Gustafsson, P. Giacomini, P. G. Natali

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Abstract

DNA methylation at the 5'-CCGG-3' sites of the HLA-DRα gene and relative flanking regions has been analyzed by Msp I and Hpa II enzymatic digestion in order to determine whether a correlation exists between DNA methylation and transcription of the HLA-DRα gene. Unexpectedly, and in contrast to the behavior of most eukaryotic genes, no positive correlation was found between hypomethylation and expression. In fact, HLA-DRα appears to be fully unmethylated at MspI/Hpa II sites in K562 cells, not expressing DR molecules, and to exhibit a high degree of methylation in Colo 38 cells, which actively transcribe the gene. The unorthodox behavior of HLA-DRα is not unique to melanoma or erythroid cells since a similar, positive correlation between methylation and expression also exists when this analysis is extended to cell lines belonging to other histotypes.

Original languageEnglish
Pages (from-to)365-369
Number of pages5
JournalEuropean Journal of Immunology
Volume16
Issue number4
Publication statusPublished - 1986

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ASJC Scopus subject areas

  • Immunology

Cite this

Gambari, R., Del Senno, L., Barbieri, R., Buzzoni, D., Gustafsson, K., Giacomini, P., & Natali, P. G. (1986). Lack of correlation between hypomethylation and expression of the HLA-DRα gene. European Journal of Immunology, 16(4), 365-369.