Lack of detectable human T-cell lymphotropic virus type I sequences in samples from multiple sclerosis patients

S. Menzo, A. Manzin, P. Bagnarelli, P. E. Varaldo, G. Grandi, G. Giuliani, G. Cazzato, M. Giacca, M. Clementi

Research output: Contribution to journalArticle

Abstract

Recently, inconclusive results have followed the early data on the possible association between multiple sclerosis (MS) and human T-cell lymphotropic virus type I (HTLV-I) infection. For this reason, we examined this hypothesis using the polymerase chain reaction (PCR) to study samples of differing origin from Italian MS patients. In particular, we developed a systematic analysis of paraffin-embedded brain white matter from histologically defined lesions of 14 MS patients using PCR and primer sets specific for HTLV-I sequences; additionally, cerebrospinal fluids (CSFs) from 12 patients and peripheral blood mononuclear cells (PBMCs) from subjects at the early and late phase of the disease were investigated for free HTLV-I virions and specific proviral sequences, respectively. In agreement with some groups who reported lack of HTLV-I sequences in PBMCs of MS patients but in clear contrast with others, we failed to detect specific viral sequences using this broad approach.

Original languageEnglish
Pages (from-to)155-161
Number of pages7
JournalJournal of Medical Virology
Volume36
Issue number3
Publication statusPublished - 1992

Keywords

  • cerebrospinal fluid
  • HLTV-1
  • multiple sclerosis
  • paraffin-embedded brain lesions
  • peripheral blood mononuclear cells

ASJC Scopus subject areas

  • Virology

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    Menzo, S., Manzin, A., Bagnarelli, P., Varaldo, P. E., Grandi, G., Giuliani, G., Cazzato, G., Giacca, M., & Clementi, M. (1992). Lack of detectable human T-cell lymphotropic virus type I sequences in samples from multiple sclerosis patients. Journal of Medical Virology, 36(3), 155-161.