Lack of detection of retroviral particles (HIAP-1) in the H9 T cell line co-cultured with thyrocytes of graves' disease

Alessandra Fierabracci, Carol Patrick Upton, Nasser Hajibagheri, Gian Franco Bottazzo

Research output: Contribution to journalArticle

Abstract

Evidence for a possible aetiopathogenetic role of endogenous and/or exogenous retroviruses (RVs) in organ- and non-organ-specific autoimmune diseases is circumstantial in both humans and animal models. Intracisternal A type particles, antigenically related to HIV, have been reported in H9 cells co-cultured with homogenates of salivary glands obtained from patients with Sjögren syndrome and with synovial fluid of patients with rheumatoid arthritis. In order to identify a possible transfer of a putative 'infective RV agent' involved in the pathogenesis of human thyroid autoimmune disease, the H9 T cell line was co-cultured not only with thyroid homogenates, but also with viable thyrocytes, both prepared from glands of patients with Graves' disease. At the end of a prolonged co-culture period (24 weeks), no RV particles could be detected by electron microscopy in the H9 cells co-cultured with both thyroid preparations. These data seem to exclude the involvement of HIAP-1 in the aetiopathogenesis of human autoimmune thyroid disease.

Original languageEnglish
Pages (from-to)457-462
Number of pages6
JournalJournal of Autoimmunity
Volume16
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • Autoimmunity
  • Electron microscopy
  • Retroviruses
  • Thyroid

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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