Lack of DNA alterations induced by orotic acid in rat liver as evaluated with two DNA unwinding methods.

C. Balbi, R. Bordone, A. Gelardi, F. Marchesini, M. Piccardo, D. S. Sarma, M. Taningher, S. Parodi

Research output: Contribution to journalArticle

Abstract

One percent orotic acid supplemented diet is a promoting treatment in the rat model of liver carcinogenesis. After treatment with this type of diet, DNA alterations were observed using alkaline sucrose gradients and alkaline elution methods. In this work we have utilized two unwinding methods for the detection of DNA fragmentation. One method is a viscosimetric method in which the rate of increase in DNA viscosity with time is related to the rate of alkaline DNA unwinding. The second method measures fluorimetrically the amount of renatured and denatured DNA after different times allowed for alkaline DNA unwinding. These two methods are very sensitive in detecting DNA breaks induced by typical alkylating agents, X-rays and H2O2. The two unwinding methods were clearly negative for the orotic acid supplemented diet. We suggest that the DNA alterations detected with alkaline sucrose gradients and alkaline elution methods, after promoting treatment with orotic acid, are probably different from the DNA breaks induced by typical alkylating agents, X-rays and H2O2.

Original languageEnglish
Pages (from-to)135-144
Number of pages10
JournalCancer Biochemistry Biophysics
Volume11
Issue number2
Publication statusPublished - Apr 1990

ASJC Scopus subject areas

  • Biophysics

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    Balbi, C., Bordone, R., Gelardi, A., Marchesini, F., Piccardo, M., Sarma, D. S., Taningher, M., & Parodi, S. (1990). Lack of DNA alterations induced by orotic acid in rat liver as evaluated with two DNA unwinding methods. Cancer Biochemistry Biophysics, 11(2), 135-144.