TY - JOUR
T1 - Lack of expression of inhibitory KIR3DL1 receptor in patients with natural killer cell-type lymphoproliferative disease of granular lymphocytes
AU - Gattazzo, Cristina
AU - Teramo, Antonella
AU - Miorin, Marta
AU - Scquizzato, Elisa
AU - Cabrelle, Anna
AU - Balsamo, Mirna
AU - Agostini, Carlo
AU - Vendrame, Elena
AU - Facco, Monica
AU - Albergoni, Maria Paola
AU - Trentin, Livio
AU - Vitale, Massimo
AU - Semenzato, Gianpietro
AU - Zambello, Renato
PY - 2010/10
Y1 - 2010/10
N2 - Background Natural killer cell-type lymphoproliferative disease of granular lymphocytes is a disorder characterized by chronic proliferation of CD3-CD16+ granular lymphocytes. By flow cytometry analysis, we previously demonstrated a dysregulation in killer immunoglobulin-like receptor (KIR) expression in natural killer cells from patients with this lymphoproliferative disease, the activating KIR receptors being mostly expressed. We also found that patients with natural killer cell-type lymphoproliferative disease of granular lymphocytes usually had KIR genotypes characterized by multiple activating KIR genes. Design and Methods We investigated the mRNA levels of the KIR3DL1 inhibitory and the related KIR3DS1 activating receptors in 15 patients with natural killer cell-type lymphoproliferative disease of granular lymphocytes and in ten controls. These genes are usually expressed when present in the genome of the Caucasian population. Results We demonstrated the complete lack of KIR3DL1 expression in most of the patients analyzed, with the receptor being expressed in 13% of patients compared to in 90% of controls (P
AB - Background Natural killer cell-type lymphoproliferative disease of granular lymphocytes is a disorder characterized by chronic proliferation of CD3-CD16+ granular lymphocytes. By flow cytometry analysis, we previously demonstrated a dysregulation in killer immunoglobulin-like receptor (KIR) expression in natural killer cells from patients with this lymphoproliferative disease, the activating KIR receptors being mostly expressed. We also found that patients with natural killer cell-type lymphoproliferative disease of granular lymphocytes usually had KIR genotypes characterized by multiple activating KIR genes. Design and Methods We investigated the mRNA levels of the KIR3DL1 inhibitory and the related KIR3DS1 activating receptors in 15 patients with natural killer cell-type lymphoproliferative disease of granular lymphocytes and in ten controls. These genes are usually expressed when present in the genome of the Caucasian population. Results We demonstrated the complete lack of KIR3DL1 expression in most of the patients analyzed, with the receptor being expressed in 13% of patients compared to in 90% of controls (P
KW - KIR3DL1 down-regulation
KW - Lymphoproliferative disease of granular lymphocytes
KW - Natural killer cells
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U2 - 10.3324/haematol.2010.023358
DO - 10.3324/haematol.2010.023358
M3 - Article
C2 - 20410181
AN - SCOPUS:77957775503
VL - 95
SP - 1722
EP - 1729
JO - Haematologica
JF - Haematologica
SN - 0390-6078
IS - 10
ER -