Lack of glucocorticoid-induced leucine zipper (GILZ) deregulates B-cell survival and results in B-cell lymphocytosis in mice

Stefano Bruscoli, Michele Biagioli, Daniele Sorcini, Tiziana Frammartino, Monica Cimino, Paolo Sportoletti, Emanuela Mazzon, Oxana Bereshchenko, Carlo Riccardi

Research output: Contribution to journalArticle

Abstract

Glucocorticoids (GC) are widely used as antiinflammatory/immunosuppressive drugs and antitumor agents in several types of lymphoma and leukemia. Therapeutic doses of GC induce growth-suppressive and cytotoxic effects on various leukocytes including B cells. Molecularmechanisms of GCaction include induction of GC target genes. Glucocorticoidinduced leucine zipper (GILZ) is a rapidly, potently, and invariably GC-induced gene. It mediates a number of GC effects, such as control of cell proliferation, differentiation, and apoptosis. Here we show that deletion of GILZ in mice leads to an accumulation of B lymphocytes in the bone marrow, blood, and lymphoid tissues. Gilz knockout (KO) mice develop a progressive nonlethalBlymphocytosis,with expansion ofB2201 cells in the bone marrowand in the periphery, dependent on increased B-cell survival. Decreased B-cell apoptosis in mice lacking GILZ correlates with increased NF-kB transcriptional activity and Bcl-2 expression. B cell-specific gilz KO mice confirmed that the effect of GILZ deletion is B-cell self-intrinsic. These results establish GILZ as an important regulator of B-cell survival and suggest that the deregulation of GILZ expression could be implicated in the pathogenesis of B-cell disorders.

Original languageEnglish
Pages (from-to)1790-1801
Number of pages12
JournalBlood
Volume126
Issue number15
DOIs
Publication statusPublished - Oct 8 2015

Fingerprint

Leucine Zippers
Lymphocytosis
Glucocorticoids
Cell Survival
B-Lymphocytes
Cells
Knockout Mice
Bone
Genes
Apoptosis
Deregulation
Lymphocytes
NF-kappa B
Cell proliferation
Lymphoid Tissue
Immunosuppressive Agents
Antineoplastic Agents
Cell Differentiation
Lymphoma
Leukemia

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology
  • Medicine(all)

Cite this

Bruscoli, S., Biagioli, M., Sorcini, D., Frammartino, T., Cimino, M., Sportoletti, P., ... Riccardi, C. (2015). Lack of glucocorticoid-induced leucine zipper (GILZ) deregulates B-cell survival and results in B-cell lymphocytosis in mice. Blood, 126(15), 1790-1801. https://doi.org/10.1182/blood-2015-03-631580

Lack of glucocorticoid-induced leucine zipper (GILZ) deregulates B-cell survival and results in B-cell lymphocytosis in mice. / Bruscoli, Stefano; Biagioli, Michele; Sorcini, Daniele; Frammartino, Tiziana; Cimino, Monica; Sportoletti, Paolo; Mazzon, Emanuela; Bereshchenko, Oxana; Riccardi, Carlo.

In: Blood, Vol. 126, No. 15, 08.10.2015, p. 1790-1801.

Research output: Contribution to journalArticle

Bruscoli, S, Biagioli, M, Sorcini, D, Frammartino, T, Cimino, M, Sportoletti, P, Mazzon, E, Bereshchenko, O & Riccardi, C 2015, 'Lack of glucocorticoid-induced leucine zipper (GILZ) deregulates B-cell survival and results in B-cell lymphocytosis in mice', Blood, vol. 126, no. 15, pp. 1790-1801. https://doi.org/10.1182/blood-2015-03-631580
Bruscoli, Stefano ; Biagioli, Michele ; Sorcini, Daniele ; Frammartino, Tiziana ; Cimino, Monica ; Sportoletti, Paolo ; Mazzon, Emanuela ; Bereshchenko, Oxana ; Riccardi, Carlo. / Lack of glucocorticoid-induced leucine zipper (GILZ) deregulates B-cell survival and results in B-cell lymphocytosis in mice. In: Blood. 2015 ; Vol. 126, No. 15. pp. 1790-1801.
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