Lack of HLA-class I antigens in human neuroblastoma cells: Analysis of its relationship to TAP and tapasin expression

M. V. Corrias, M. Occhino, M. Croce, A. De Ambrosis, M. P. Pistillo, P. Bocca, V. Pistoia, S. Ferrini

Research output: Contribution to journalArticle

Abstract

We studied the constitutive and the interferon (IFN)-γ-induced expression of HLA class I antigen heavy chain, β2-microglobulin (β2m), TAP-1, TAP-2 and tapasin in a panel of eleven neuroblastoma cell lines. Surface expression of HLA class I antigens was low in eight out of eight neuroblastoma cell lines bearing MYC-N amplification and/or 1p deletion, while two out of three neuroblastoma cell lines lacking these genetic alterations showed normal expression. IFN-γ treatment restored HLA class I antigen surface expression in all neuroblastoma cell lines. Eight out of 11 neuroblastoma cell lines did not express TAP-1 mRNA and three of them also lacked TAP-2 mRNA. β2 m mRNA was barely detectable or absent in five neuroblastoma cell lines, while tapasin mRNA was always expressed. IFN-γ upregulated the expression of HLA class I heavy chain, β2 m, TAP-1, TAP-2 and tapasin, as detected at mRNA or protein level. Post-transcriptional events were involved in altered TAP-1 and β2 m expression in one peculiar neuroblastoma cell line. These data indicate that multiple mechanisms play a role in the HLA class I antigen-deficient phenotype of human neuroblastoma.

Original languageEnglish
Pages (from-to)110-117
Number of pages8
JournalTissue Antigens
Volume57
Issue number2
DOIs
Publication statusPublished - 2001

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Keywords

  • HLA class I
  • Interferon-γ
  • Neuroblastoma
  • TAP
  • Tapasin

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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