Abstract
The ability of human recombinant interferon-α2a (IFNα2a) to induce the expression of cyclin-dependent kinase inhibitors p21(waf1) and p27(kip1) consequent to signal transducers and activators of transcription (STAT) protein activation was investigated in six human melanoma cell lines with different susceptibilities to the antiproliferative effect of the cytokine. All the cell lines expressed IFNα and IFNα/β receptors. Exposure for 24 h to IFNα2a markedly enhanced the nuclear expression of STAT1 and STAT2 proteins in all the cell lines. However, no induction of p21(waf1) or p27(kip1) was consistently observed. Overall, results from the study suggest that the induction of such cyclin-dependent kinase inhibitors is not a major mechanism for the antiproliferative effect of IFNα2a, at least in human melanoma cell lines.
| Original language | English |
|---|---|
| Pages (from-to) | 457-463 |
| Number of pages | 7 |
| Journal | Melanoma Research |
| Volume | 9 |
| Issue number | 5 |
| Publication status | Published - 1999 |
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Keywords
- Cell growth
- Cyclin-dependent kinases
- Interferon-α2a
- STAT proteins
ASJC Scopus subject areas
- Cancer Research
- Dermatology
Cite this
Lack of p21(waf1) and p27(kip1) protein induction by interferon-α2a in human melanoma cell lines. / Bearzatto, A.; Orlandi, L.; De Marco, C.; Daidone, M. G.; Zaffaroni, N.
In: Melanoma Research, Vol. 9, No. 5, 1999, p. 457-463.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lack of p21(waf1) and p27(kip1) protein induction by interferon-α2a in human melanoma cell lines
AU - Bearzatto, A.
AU - Orlandi, L.
AU - De Marco, C.
AU - Daidone, M. G.
AU - Zaffaroni, N.
PY - 1999
Y1 - 1999
N2 - The ability of human recombinant interferon-α2a (IFNα2a) to induce the expression of cyclin-dependent kinase inhibitors p21(waf1) and p27(kip1) consequent to signal transducers and activators of transcription (STAT) protein activation was investigated in six human melanoma cell lines with different susceptibilities to the antiproliferative effect of the cytokine. All the cell lines expressed IFNα and IFNα/β receptors. Exposure for 24 h to IFNα2a markedly enhanced the nuclear expression of STAT1 and STAT2 proteins in all the cell lines. However, no induction of p21(waf1) or p27(kip1) was consistently observed. Overall, results from the study suggest that the induction of such cyclin-dependent kinase inhibitors is not a major mechanism for the antiproliferative effect of IFNα2a, at least in human melanoma cell lines.
AB - The ability of human recombinant interferon-α2a (IFNα2a) to induce the expression of cyclin-dependent kinase inhibitors p21(waf1) and p27(kip1) consequent to signal transducers and activators of transcription (STAT) protein activation was investigated in six human melanoma cell lines with different susceptibilities to the antiproliferative effect of the cytokine. All the cell lines expressed IFNα and IFNα/β receptors. Exposure for 24 h to IFNα2a markedly enhanced the nuclear expression of STAT1 and STAT2 proteins in all the cell lines. However, no induction of p21(waf1) or p27(kip1) was consistently observed. Overall, results from the study suggest that the induction of such cyclin-dependent kinase inhibitors is not a major mechanism for the antiproliferative effect of IFNα2a, at least in human melanoma cell lines.
KW - Cell growth
KW - Cyclin-dependent kinases
KW - Interferon-α2a
KW - STAT proteins
UR - http://www.scopus.com/inward/record.url?scp=0032586031&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032586031&partnerID=8YFLogxK
M3 - Article
VL - 9
SP - 457
EP - 463
JO - Melanoma Research
JF - Melanoma Research
SN - 0960-8931
IS - 5
ER -