Lack of selective Vβ deletion in CD4+ or CD8+ T lymphocytes and functional integrity of T-cell repertoire during acute HIV syndrome

A. Cossarizza, C. Ortolani, C. Mussini, G. Guaraldi, N. Mongiardo, V. Borghi, D. Barbieri, E. Bellesia, M. G. Franceschini, B. De Rienzo, C. Franceschi

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To study the Vβ T-cell repertoire in peripheral blood lymphocytes (PBL) during acute HIV syndrome by using several anti-Vβ monoclonal antibodies (MAb) and to analyse its functionality by stimulating PBL with superantigens (SAg) such as Staphylococcus aureus enterotoxins. Methods: Cytofluorimetric analysis of Vβ T-cell-receptor expression was performed on PBL from eight patients with symptomatic, acute HIV-1 primary infection, showing a dramatic decrease of CD4+ PBL accompanied by a marked increase in activated/memory CD8+ T cells, and on 12 age- and sex-matched healthy controls. PBL were then isolated, stimulated with different SAg, anti-CD3 MAb or phytohaemagglutinin and cultured for 3 days. PBL capability to progress through cell cycle was studied by the classic cytofluorimetric method of bromodeoxyuridine incorporation and DNA staining with propidium iodide. Results: Despite the presence of a few expansions of some Vβ families among CD8+ T lymphocytes, no gross alterations in T-cell repertoire were present in patients with acute HIV syndrome. Its functionality was maintained overall, as PBL responsiveness to SAg was well preserved. Interestingly, all CD8+ T cells, although bearing different Vβ T-cell receptors, expressed marked signs of activation, i.e., CD45RO, CD38 and major histocompatibility complex class II molecules, and also high amounts of CD11a and CD18. Conclusions: Our data suggest, at least in the early phases and in the acute form of the infection, that HIV is not likely to act as a SAg. However, further studies are needed to analyse other sites, such as lymph nodes, where HIV could exert other, significant effects, and to study the expression of other Vβ families than those investigated here.

Original languageEnglish
Pages (from-to)547-553
Number of pages7
JournalAIDS (London, England)
Volume9
Issue number6
Publication statusPublished - 1995

Keywords

  • Acute HIV syndrome
  • AIDS
  • CD45
  • Cell adhesion molecules
  • Cytokines
  • HIV
  • Interleukin-6
  • T-cell repertoire
  • Tumour necrosis factor-α
  • Vβ T-cell receptor

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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