Lack of type 1 and type 2A myosin heavy chain isoforms in rat slow muscle regenerating during chronic nerve block

Menotti Midrio, Daniela Danieli-Betto, Alessandra Esposito, Aram Megighian, Ugo Carraro, Claudia Catani, Katia Rossini

Research output: Contribution to journalArticle

Abstract

The degeneration-regeneration process was induced by bupivacaine injection in innervated, denervated, and nerve-blocked rat soleus muscles. Nerve block was obtained by superfusion of the sciatic nerve with tetrodotoxin (TTX). Two weeks after bupivacaine injection, immunohistochemical and electrophoretical analyses showed the presence of type 1 myosin heavy chain (MHC) only in innervated regenerated muscles, type 2A in innervated and denervated, but not in TTX-paralyzed muscles, and type 2X under all experimental conditions. The presence of type 1 MHC in the innervated, and its absence in both denervated and TTX-paralyzed muscles were also verified immunohistochemically 1 week after bupivacaine injection. It is concluded that the nerve impulses play a determinant role in the expression of 1 and 2A MHC isoforms in the innervated regenerating muscle. The possible causes of the absence of the type 2A MHC isoform in the TTX-paralyzed muscles are discussed.

Original languageEnglish
Pages (from-to)226-232
Number of pages7
JournalMuscle and Nerve
Volume21
Issue number2
DOIs
Publication statusPublished - 1998

Keywords

  • Muscle differentiation
  • Myosin heavy chain
  • Nerve activity
  • Neurotrophic factors

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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