TY - JOUR
T1 - Lactobacillus rhamnosus GG affects the BDNF system in brain samples of wistar rats with pepsin-trypsin-digested gliadin (PTG)-induced enteropathy
AU - Orlando, Antonella
AU - Chimienti, Guglielmina
AU - Lezza, Angela Maria Serena
AU - Pesce, Vito
AU - Gigante, Isabella
AU - D’attoma, Benedetta
AU - Russo, Francesco
N1 - Funding Information:
Funding: This research was funded by the Italian Ministry of Health (Grant n.45-24.01.2017) and the University of Bari (Grant 2015/16 and Grant 2017/18).
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/3
Y1 - 2020/3
N2 - Celiac disease (CD) presents as chronic low-grade inflammation of the small intestine often characterized by psychiatric comorbidities. The brain-derived neurotrophic factor (BDNF), which we have shown to be reduced in the serum of CD patients, acts as the bridge between immune activation and the nervous system adaptive response. Since Lactobacillus has been shown to upregulate BDNF, this study aimed to evaluate whether the administration of Lactobacillus rhamnosus GG (L.GG) could positively affect the brain BDNF system in rats mimicking the CD lesions. Data have shown that the administration of pepsin-trypsin digested gliadin (PTG) and L.GG alter the levels of mature BDNF (mBDNF), as evaluated by Western blotting. PTG provoked a reduction of mBDNF compared to controls, and a compensatory increase of its receptor TrkB. L.GG induced a slight positive effect on mBDNF levels under normal conditions, while it was able to rescue the PTG-induced reduced expression of mBDNF. The curative effect of L.GG was finely tuned, accompanied by the reduction of TrkB, probably to avoid the effect of excessive BDNF.
AB - Celiac disease (CD) presents as chronic low-grade inflammation of the small intestine often characterized by psychiatric comorbidities. The brain-derived neurotrophic factor (BDNF), which we have shown to be reduced in the serum of CD patients, acts as the bridge between immune activation and the nervous system adaptive response. Since Lactobacillus has been shown to upregulate BDNF, this study aimed to evaluate whether the administration of Lactobacillus rhamnosus GG (L.GG) could positively affect the brain BDNF system in rats mimicking the CD lesions. Data have shown that the administration of pepsin-trypsin digested gliadin (PTG) and L.GG alter the levels of mature BDNF (mBDNF), as evaluated by Western blotting. PTG provoked a reduction of mBDNF compared to controls, and a compensatory increase of its receptor TrkB. L.GG induced a slight positive effect on mBDNF levels under normal conditions, while it was able to rescue the PTG-induced reduced expression of mBDNF. The curative effect of L.GG was finely tuned, accompanied by the reduction of TrkB, probably to avoid the effect of excessive BDNF.
KW - Brain-derived neurotrophic factor
KW - Celiac disease
KW - Gliadin
KW - Lactobacillus rhamnosus GG
KW - Probiotics
KW - TrkB receptor
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U2 - 10.3390/nu12030629
DO - 10.3390/nu12030629
M3 - Article
C2 - 32120967
AN - SCOPUS:85080945289
VL - 12
JO - Nutrients
JF - Nutrients
SN - 2072-6643
IS - 3
M1 - 629
ER -