Lactoferrin binding sites and nuclear localization in K562(S) cells

Cecilia Carré, Giovanna Bianchi-Scarrà, Mario Sirito, Marco Musso, Roberto Ravazzolo

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Lactoterrin, a single chain cationic glycoprotein, present in the secondary granules of neutrophils, acts as a negative teed bark regulator of myelopoiesis. Specific receptors tor lactoferrin were detected on the surface ot different hematopoietic cell types. The influence of lactoferrin on cell growth in culture has been reported. Interactions of lactoferrin with DNA were also demonstrated. In the present paper we confirm the presence of lactoferrin specific binding sites on K562 cells and we estimate the number of binding sites and the dissociation constant. By Western blotting analysis performed on K562 lysates we find a band uf about 120 kUa responsible for specific binding of lactoferrin. We also show that lactoferrin, after binding at the cell surface, is internalized in a temperature dependent way and is immunologkally detectable as a DNA-linked protein in nuclearextracts.

Original languageEnglish
Pages (from-to)477-482
Number of pages6
JournalJournal of Cellular Physiology
Volume153
Issue number3
Publication statusPublished - Dec 1992

Fingerprint

Lactoferrin
K562 Cells
Binding Sites
Myelopoiesis
DNA
Cell growth
Glycoproteins
Cell culture
Neutrophils
Cell Count
Western Blotting
Temperature
Growth
Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Carré, C., Bianchi-Scarrà, G., Sirito, M., Musso, M., & Ravazzolo, R. (1992). Lactoferrin binding sites and nuclear localization in K562(S) cells. Journal of Cellular Physiology, 153(3), 477-482.

Lactoferrin binding sites and nuclear localization in K562(S) cells. / Carré, Cecilia; Bianchi-Scarrà, Giovanna; Sirito, Mario; Musso, Marco; Ravazzolo, Roberto.

In: Journal of Cellular Physiology, Vol. 153, No. 3, 12.1992, p. 477-482.

Research output: Contribution to journalArticle

Carré, C, Bianchi-Scarrà, G, Sirito, M, Musso, M & Ravazzolo, R 1992, 'Lactoferrin binding sites and nuclear localization in K562(S) cells', Journal of Cellular Physiology, vol. 153, no. 3, pp. 477-482.
Carré C, Bianchi-Scarrà G, Sirito M, Musso M, Ravazzolo R. Lactoferrin binding sites and nuclear localization in K562(S) cells. Journal of Cellular Physiology. 1992 Dec;153(3):477-482.
Carré, Cecilia ; Bianchi-Scarrà, Giovanna ; Sirito, Mario ; Musso, Marco ; Ravazzolo, Roberto. / Lactoferrin binding sites and nuclear localization in K562(S) cells. In: Journal of Cellular Physiology. 1992 ; Vol. 153, No. 3. pp. 477-482.
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