Lactulose and mannitol intestinal permeability detected by capillary electrophoresis

Rita Paroni, Isabella Fermo, Laura Molteni, Laura Folini, Matteo Rocco Pastore, Andrea Mosca, Emanuele Bosi

Research output: Contribution to journalArticlepeer-review

Abstract

Aim of this study was to set up a method by capillary electrophoresis to detect lactulose and mannitol in urine after an oral load, and to estimate the intestinal permeability in controls and in type I diabetes patients. The underivatized carbohydrates were monitored by indirect UV detection using sorbate, cetyltrimethylammonium bromide and LiOH as background electrolyte. Urines were purified by solid phase extraction, shaken with cation exchange resin, filtered and analysed. Carbohydrates migrated in a and Mr. Controls (n = 33) and patients (n = 23) had an excretion ratio lactulose/mannitol 0.025 (0.018-0.051) and 0.067 (0.050-0.127), respectively (p <0.01, median, interquartile range).

Original languageEnglish
Pages (from-to)183-187
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume834
Issue number1-2
DOIs
Publication statusPublished - Apr 13 2006

Keywords

  • Capillary electrophoresis
  • Intestinal permeability
  • Lactulose
  • Mannitol
  • Urine

ASJC Scopus subject areas

  • Biochemistry

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