Laminin inhibits the adhesion of a murine tumor of macrophage origin

Raffaella Giavazzi, Lance Liotta, Ian Hart

Research output: Contribution to journalArticle

Abstract

The adhesive behavior of the M5076 reticulum cell sarcoma, a highly metastatic murine tumor of macrophage origin, was investigated in vitro both in the presence and in the absence of purified exogeneous laminin. Although laminin enhanced the adhesion of other murine cell lines to collagen-coated and plastic surfaces, it reduced the attachment to both substrates of M5076 cells, peritoneal macrophages and the macrophage cell line WEHI-3. Thus, the inhibition appeared to be related to the macrophage nature of the M5076 tumor. The effects of laminin were reversible and did not cause loss of viability or functional capacity of the M5076 cells. Laminin appeared to exert this inhibitory effect by binding to the substrates rather than binding to the cells. These studies indicate that laminin, a glycoprotein from the basement membrane, may either stimulate or inhibit cell attachment, depending on the type of cell.

Original languageEnglish
Pages (from-to)315-322
Number of pages8
JournalExperimental Cell Research
Volume140
Issue number2
DOIs
Publication statusPublished - 1982

ASJC Scopus subject areas

  • Cell Biology

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