LANCL2 is necessary for abscisic acid binding and signaling in human granulocytes and in rat insulinoma cells

Laura Sturla, Chiara Fresia, Lucrezia Guida, Santina Bruzzone, Sonia Scarfi, Cesare Usai, Floriana Fruscione, Mirko Magnone, Enrico Millo, Giovanna Basile, Alessia Grozio, Emanuela Jacchetti, Marcello Allegretti, Antonio De Flora, Elena Zocchi

Research output: Contribution to journalArticlepeer-review


Abscisic acid (ABA) is a plant hormone regulating fundamental physiological functions in plants, such as response to abiotic stress. Recently,ABAwas shown to be produced and released by human granulocytes, by insulin-producing rat insulinoma cells, and by human and murine pancreatic β cells. ABA autocrinally stimulates the functional activities specific for each cell type through a receptor-operated signal transduction pathway, sequentially involving a pertussis toxin-sensitive receptor/Gprotein complex, cAMP, CD38-produced cADP-ribose and intracellular calcium. Here we show that the lanthionine synthetase C-like protein LANCL2 is required for ABA binding on the membrane of human granulocytes and that LANCL2 is necessary for transduction of the ABA signal into the cell-specific functional responses in granulocytes and in rat insulinoma cells. Co-expression of LANCL2 and CD38 in the human HeLa cell line reproduces the ABA-signaling pathway. Results obtained with granulocytes and CD38+/LANCL2+ HeLa transfected with a chimeric G-protein (Gαq/i) suggest that the pertussis toxin-sensitive G-protein coupled to LANCL2 is a Gi. Identification of LANCL2 as a critical component of the ABA-sensing protein complex will enable the screening of synthetic ABA antagonists as prospective new anti-inflammatory and anti-diabetic agents.

Original languageEnglish
Pages (from-to)28045-28057
Number of pages13
JournalJournal of Biological Chemistry
Issue number41
Publication statusPublished - Oct 9 2009

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology


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