TY - JOUR
T1 - LANCL2 is necessary for abscisic acid binding and signaling in human granulocytes and in rat insulinoma cells
AU - Sturla, Laura
AU - Fresia, Chiara
AU - Guida, Lucrezia
AU - Bruzzone, Santina
AU - Scarfi, Sonia
AU - Usai, Cesare
AU - Fruscione, Floriana
AU - Magnone, Mirko
AU - Millo, Enrico
AU - Basile, Giovanna
AU - Grozio, Alessia
AU - Jacchetti, Emanuela
AU - Allegretti, Marcello
AU - De Flora, Antonio
AU - Zocchi, Elena
PY - 2009/10/9
Y1 - 2009/10/9
N2 - Abscisic acid (ABA) is a plant hormone regulating fundamental physiological functions in plants, such as response to abiotic stress. Recently,ABAwas shown to be produced and released by human granulocytes, by insulin-producing rat insulinoma cells, and by human and murine pancreatic β cells. ABA autocrinally stimulates the functional activities specific for each cell type through a receptor-operated signal transduction pathway, sequentially involving a pertussis toxin-sensitive receptor/Gprotein complex, cAMP, CD38-produced cADP-ribose and intracellular calcium. Here we show that the lanthionine synthetase C-like protein LANCL2 is required for ABA binding on the membrane of human granulocytes and that LANCL2 is necessary for transduction of the ABA signal into the cell-specific functional responses in granulocytes and in rat insulinoma cells. Co-expression of LANCL2 and CD38 in the human HeLa cell line reproduces the ABA-signaling pathway. Results obtained with granulocytes and CD38+/LANCL2+ HeLa transfected with a chimeric G-protein (Gαq/i) suggest that the pertussis toxin-sensitive G-protein coupled to LANCL2 is a Gi. Identification of LANCL2 as a critical component of the ABA-sensing protein complex will enable the screening of synthetic ABA antagonists as prospective new anti-inflammatory and anti-diabetic agents.
AB - Abscisic acid (ABA) is a plant hormone regulating fundamental physiological functions in plants, such as response to abiotic stress. Recently,ABAwas shown to be produced and released by human granulocytes, by insulin-producing rat insulinoma cells, and by human and murine pancreatic β cells. ABA autocrinally stimulates the functional activities specific for each cell type through a receptor-operated signal transduction pathway, sequentially involving a pertussis toxin-sensitive receptor/Gprotein complex, cAMP, CD38-produced cADP-ribose and intracellular calcium. Here we show that the lanthionine synthetase C-like protein LANCL2 is required for ABA binding on the membrane of human granulocytes and that LANCL2 is necessary for transduction of the ABA signal into the cell-specific functional responses in granulocytes and in rat insulinoma cells. Co-expression of LANCL2 and CD38 in the human HeLa cell line reproduces the ABA-signaling pathway. Results obtained with granulocytes and CD38+/LANCL2+ HeLa transfected with a chimeric G-protein (Gαq/i) suggest that the pertussis toxin-sensitive G-protein coupled to LANCL2 is a Gi. Identification of LANCL2 as a critical component of the ABA-sensing protein complex will enable the screening of synthetic ABA antagonists as prospective new anti-inflammatory and anti-diabetic agents.
UR - http://www.scopus.com/inward/record.url?scp=70350504374&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350504374&partnerID=8YFLogxK
U2 - 10.1074/jbc.M109.035329
DO - 10.1074/jbc.M109.035329
M3 - Article
C2 - 19667068
AN - SCOPUS:70350504374
VL - 284
SP - 28045
EP - 28057
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 41
ER -