Lanreotide-induced modulation of 5-fluorouracil or mitomycin C cytotoxicity in human colon cancer cell lines: A preclinical study

A. Tesei, L. Ricotti, F. De Paola, C. Casini-Raggi, F. Barzanti, G. L. Frassineti, W. Zoli

Research output: Contribution to journalArticle

Abstract

The effect on growth of the long-acting somatostatin analogue lanreotide (LAN), alone or in combination with 5-fluorouracil (5-FU) and mitomycin C (MIT), was investigated in three human colon cancer lines. Cell survival inhibition induced by LAN alone, as evaluated by sulforhodamine B assay, ranged from 20% to 40% as a function of cell line and concentration. The IC50, the concentration inhibiting cell survival by 50%, was never reached. The antiproliferative effect produced by a 48h exposure to 5-FU or MIT was synergistically enhanced in all cell lines by a subsequent 48h exposure to LAN. The synergistic interaction was not related to specific cell cycle perturbations or to the somatostatin receptor 2 (sst2) mRNA abundance. In conclusion, our study seems to indicate that LAN is a potentially useful modulating agent for enhancing 5-FU and MIT activity in colorectal cancer patients.

Original languageEnglish
Pages (from-to)421-430
Number of pages10
JournalJournal of Chemotherapy
Volume12
Issue number5
Publication statusPublished - 2000

Keywords

  • 5-fluorouracil
  • Chemosensitivity
  • Colon cancer cell lines
  • Drug interactions
  • Lanreotide
  • Mitomycin C
  • Somatostatin receptors

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)

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