Large granular lymphocytes from patients with expanded LGL populations acquire cytotoxic functions and release lymphokines upon in vitro activation

V. Pistoia, E. F. Prasthofer, A. B. Tilden, J. C. Barton, M. Ferrarini, C. E. Grossi, K. Zuckerman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The phenotypic and functional features of purified large granular lymphocytes (LGL) from ten patients with LGL population expansions and cytopenias are described. The predominant LGL phenotypes were T3+, T8+, Leu-11+/-; however, in two patients, LGL expressed a T3-, Leu-11+ phenotype. Variable combinations of other LGL markers (OKM1, Leu-7), and HLA-DR were detected in individual cases. In nine of ten cases, freshly isolated LGL did not exert cytolytic activity for K562 target cells, but purified LGL cultured in the presence of recombinant interleukin 2 (rIL2) acquired potent cytotoxic activity in all cases tested. LGL did not proliferate in response to phytohemagglutinin (PHA). However, LGL released variable amounts of IL2 and γ-interferon (γ-IFN) after PHA stimulation. In some cases, stimulation of fresh LGL with recombinant IL2 induced production of γ-IFN. No correlation was found between the functional capabilities and the original phenotype of the expanded LGL populations.

Original languageEnglish
Pages (from-to)1095-1100
Number of pages6
JournalBlood
Volume68
Issue number5
Publication statusPublished - 1986

Fingerprint

Lymphocytes
Lymphokines
Chemical activation
Population
Interleukin-2
Phytohemagglutinins
Phenotype
In Vitro Techniques
K562 Cells
HLA-DR Antigens
Interferons

ASJC Scopus subject areas

  • Hematology

Cite this

Pistoia, V., Prasthofer, E. F., Tilden, A. B., Barton, J. C., Ferrarini, M., Grossi, C. E., & Zuckerman, K. (1986). Large granular lymphocytes from patients with expanded LGL populations acquire cytotoxic functions and release lymphokines upon in vitro activation. Blood, 68(5), 1095-1100.

Large granular lymphocytes from patients with expanded LGL populations acquire cytotoxic functions and release lymphokines upon in vitro activation. / Pistoia, V.; Prasthofer, E. F.; Tilden, A. B.; Barton, J. C.; Ferrarini, M.; Grossi, C. E.; Zuckerman, K.

In: Blood, Vol. 68, No. 5, 1986, p. 1095-1100.

Research output: Contribution to journalArticle

Pistoia, V, Prasthofer, EF, Tilden, AB, Barton, JC, Ferrarini, M, Grossi, CE & Zuckerman, K 1986, 'Large granular lymphocytes from patients with expanded LGL populations acquire cytotoxic functions and release lymphokines upon in vitro activation', Blood, vol. 68, no. 5, pp. 1095-1100.
Pistoia, V. ; Prasthofer, E. F. ; Tilden, A. B. ; Barton, J. C. ; Ferrarini, M. ; Grossi, C. E. ; Zuckerman, K. / Large granular lymphocytes from patients with expanded LGL populations acquire cytotoxic functions and release lymphokines upon in vitro activation. In: Blood. 1986 ; Vol. 68, No. 5. pp. 1095-1100.
@article{7f14bfdc4b1e482a80aa65bcae6b953a,
title = "Large granular lymphocytes from patients with expanded LGL populations acquire cytotoxic functions and release lymphokines upon in vitro activation",
abstract = "The phenotypic and functional features of purified large granular lymphocytes (LGL) from ten patients with LGL population expansions and cytopenias are described. The predominant LGL phenotypes were T3+, T8+, Leu-11+/-; however, in two patients, LGL expressed a T3-, Leu-11+ phenotype. Variable combinations of other LGL markers (OKM1, Leu-7), and HLA-DR were detected in individual cases. In nine of ten cases, freshly isolated LGL did not exert cytolytic activity for K562 target cells, but purified LGL cultured in the presence of recombinant interleukin 2 (rIL2) acquired potent cytotoxic activity in all cases tested. LGL did not proliferate in response to phytohemagglutinin (PHA). However, LGL released variable amounts of IL2 and γ-interferon (γ-IFN) after PHA stimulation. In some cases, stimulation of fresh LGL with recombinant IL2 induced production of γ-IFN. No correlation was found between the functional capabilities and the original phenotype of the expanded LGL populations.",
author = "V. Pistoia and Prasthofer, {E. F.} and Tilden, {A. B.} and Barton, {J. C.} and M. Ferrarini and Grossi, {C. E.} and K. Zuckerman",
year = "1986",
language = "English",
volume = "68",
pages = "1095--1100",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "5",

}

TY - JOUR

T1 - Large granular lymphocytes from patients with expanded LGL populations acquire cytotoxic functions and release lymphokines upon in vitro activation

AU - Pistoia, V.

AU - Prasthofer, E. F.

AU - Tilden, A. B.

AU - Barton, J. C.

AU - Ferrarini, M.

AU - Grossi, C. E.

AU - Zuckerman, K.

PY - 1986

Y1 - 1986

N2 - The phenotypic and functional features of purified large granular lymphocytes (LGL) from ten patients with LGL population expansions and cytopenias are described. The predominant LGL phenotypes were T3+, T8+, Leu-11+/-; however, in two patients, LGL expressed a T3-, Leu-11+ phenotype. Variable combinations of other LGL markers (OKM1, Leu-7), and HLA-DR were detected in individual cases. In nine of ten cases, freshly isolated LGL did not exert cytolytic activity for K562 target cells, but purified LGL cultured in the presence of recombinant interleukin 2 (rIL2) acquired potent cytotoxic activity in all cases tested. LGL did not proliferate in response to phytohemagglutinin (PHA). However, LGL released variable amounts of IL2 and γ-interferon (γ-IFN) after PHA stimulation. In some cases, stimulation of fresh LGL with recombinant IL2 induced production of γ-IFN. No correlation was found between the functional capabilities and the original phenotype of the expanded LGL populations.

AB - The phenotypic and functional features of purified large granular lymphocytes (LGL) from ten patients with LGL population expansions and cytopenias are described. The predominant LGL phenotypes were T3+, T8+, Leu-11+/-; however, in two patients, LGL expressed a T3-, Leu-11+ phenotype. Variable combinations of other LGL markers (OKM1, Leu-7), and HLA-DR were detected in individual cases. In nine of ten cases, freshly isolated LGL did not exert cytolytic activity for K562 target cells, but purified LGL cultured in the presence of recombinant interleukin 2 (rIL2) acquired potent cytotoxic activity in all cases tested. LGL did not proliferate in response to phytohemagglutinin (PHA). However, LGL released variable amounts of IL2 and γ-interferon (γ-IFN) after PHA stimulation. In some cases, stimulation of fresh LGL with recombinant IL2 induced production of γ-IFN. No correlation was found between the functional capabilities and the original phenotype of the expanded LGL populations.

UR - http://www.scopus.com/inward/record.url?scp=0022809276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022809276&partnerID=8YFLogxK

M3 - Article

C2 - 3490286

AN - SCOPUS:0022809276

VL - 68

SP - 1095

EP - 1100

JO - Blood

JF - Blood

SN - 0006-4971

IS - 5

ER -