Large-scale manufacture and characterization of a lentiviral vector produced for clinical Ex vivo gene therapy application

Otto Wilhelm Merten, Sabine Charrier, Nicolas Laroudie, Sylvain Fauchille, Céline Dugué, Christine Jenny, Muriel Audit, Maria Antonietta Zanta-Boussif, Hélène Chautard, Marina Radrizzani, Giuliana Vallanti, Luigi Naldini, Patricia Noguiez-Hellin, Anne Galy

Research output: Contribution to journalArticle

Abstract

From the perspective of a pilot clinical gene therapy trial for Wiskott-Aldrich syndrome (WAS), we implemented a process to produce a lentiviral vector under good manufacturing practices (GMP). The process is based on the transient transfection of 293T cells in Cell Factory stacks, scaled up to harvest 50 liters of viral stock per batch, followed by purification of the vesicular stomatitis virus glycoprotein-pseudotyped particles through several membrane-based and chromatographic steps. The process leads to a 200-fold volume concentration and an approximately 3-log reduction in protein and DNA contaminants. An average yield of 13% of infectious particles was obtained in six full-scale preparations. The final product contained low levels of contaminants such as simian virus 40 large T antigen or E1A sequences originating from producer cells. Titers as high as 2×109 infectious particles per milliliter were obtained, generating up to 6×1011 infectious particles per batch. The purified WAS vector was biologically active, efficiently expressing the genetic insert in WAS protein-deficient B cell lines and transducing CD34+ cells. The vector introduced 0.3-1 vector copy per cell on average in CD34+ cells when used at the concentration of 108 infectious particles per milliliter, which is comparable to preclinical preparations. There was no evidence of cellular toxicity. These results show the implementation of large-scale GMP production, purification, and control of advanced HIV-1-derived lentiviral technology. Results obtained with the WAS vector provide the initial manufacturing and quality control benchmarking that should be helpful to further development and clinical applications.

Original languageEnglish
Pages (from-to)343-356
Number of pages14
JournalHuman Gene Therapy
Volume22
Issue number3
DOIs
Publication statusPublished - Mar 1 2011

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ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Merten, O. W., Charrier, S., Laroudie, N., Fauchille, S., Dugué, C., Jenny, C., Audit, M., Zanta-Boussif, M. A., Chautard, H., Radrizzani, M., Vallanti, G., Naldini, L., Noguiez-Hellin, P., & Galy, A. (2011). Large-scale manufacture and characterization of a lentiviral vector produced for clinical Ex vivo gene therapy application. Human Gene Therapy, 22(3), 343-356. https://doi.org/10.1089/hum.2010.060