Large-scale procedure for the purification of fibronectin domains

Laura Borsi, Patrizia Castellani, Enrica Balza, Annalisa Siri, Caterina Pellecchia, Fiorella De Scalzi, Luciano Zardi

Research output: Contribution to journalArticlepeer-review

Abstract

Human plasma fibronectin is composed of at least seven distinct domains, with affinities for different macromolecules and cell surfaces. Here we describe in detail a simple high-yield procedure for the purification of large amounts of fibronectin domains. This involves thermolysin digestion of the fibronectin molecule followed by the purification of the domain using mainly hydroxyapatite chromatography columns. This procedure represents a great simplification over those previously reported.

Original languageEnglish
Pages (from-to)335-345
Number of pages11
JournalAnalytical Biochemistry
Volume155
Issue number2
DOIs
Publication statusPublished - 1986

Keywords

  • chromatography
  • fibronectin structure
  • hydroxyapatite
  • proteins
  • purification
  • thermolysin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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