Large-Scale Survey of Human Enteroviruses in Wastewater Treatment Plants of a Metropolitan Area of Southern Italy

Francesca Pennino, Antonio Nardone, Paolo Montuori, Sara Aurino, Ida Torre, Andrea Battistone, Roberto Delogu, Gabriele Buttinelli, Stefano Fiore, Concetta Amato, Maria Triassi

Research output: Contribution to journalArticle

Abstract

Human enteroviruses (HEVs) occur in high concentrations in wastewater and can contaminate receiving environmental waters, constituting a major cause of acute waterborne disease worldwide. In this study, we investigated the relative abundance, occurrence, and seasonal distribution of polio and other enteroviruses at three wastewater treatment plants (WWTPs) in Naples, Southern Italy, from January 2010 to December 2014. Influent and effluent samples from the three WWTPs were collected monthly. One hundred and sixty-one of the 731 wastewater samples collected (22.0%) before and after water treatment were CPE positive on RD cells; while no samples were positive on L20B cells from any WWTPs. Among the 140 non-polio enterovirus isolated from inlet sewage, 69.3% were Coxsackieviruses type B and 30.7% were Echoviruses. Among these, CVB3 and CVB5 were most prevalent, followed by CVB4 and Echo6. The twenty-one samples tested after treatment contained 6 CVB4, 5 CVB3, 3 Echo11, and 2 Echo6; while other serotypes were isolated less frequently. Data on viral detection in treated effluents of WWTPs confirmed the potential environmental contamination by HEVs and could be useful to establish standards for policies on wastewater management.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalFood and Environmental Virology
Volume10
Issue number2
DOIs
Publication statusPublished - Jun 2018

Fingerprint

Human enterovirus
Enterovirus
Waste Water
wastewater treatment
Italy
wastewater
effluents
Human Enterovirus B
waterborne diseases
sampling
water treatment
sewage
serotypes
pollution
cells
Water Purification
Poliomyelitis
Acute Disease
Sewage
Surveys and Questionnaires

Keywords

  • Enterovirus/isolation & purification
  • Enterovirus Infections/virology
  • Humans
  • Italy
  • Poliomyelitis/virology
  • Poliovirus/isolation & purification
  • Sewage/virology
  • Surveys and Questionnaires
  • Waste Water/virology
  • Water Purification/methods

Cite this

Large-Scale Survey of Human Enteroviruses in Wastewater Treatment Plants of a Metropolitan Area of Southern Italy. / Pennino, Francesca; Nardone, Antonio; Montuori, Paolo; Aurino, Sara; Torre, Ida; Battistone, Andrea; Delogu, Roberto; Buttinelli, Gabriele; Fiore, Stefano; Amato, Concetta; Triassi, Maria.

In: Food and Environmental Virology, Vol. 10, No. 2, 06.2018, p. 187-192.

Research output: Contribution to journalArticle

Pennino, F, Nardone, A, Montuori, P, Aurino, S, Torre, I, Battistone, A, Delogu, R, Buttinelli, G, Fiore, S, Amato, C & Triassi, M 2018, 'Large-Scale Survey of Human Enteroviruses in Wastewater Treatment Plants of a Metropolitan Area of Southern Italy', Food and Environmental Virology, vol. 10, no. 2, pp. 187-192. https://doi.org/10.1007/s12560-017-9331-3
Pennino, Francesca ; Nardone, Antonio ; Montuori, Paolo ; Aurino, Sara ; Torre, Ida ; Battistone, Andrea ; Delogu, Roberto ; Buttinelli, Gabriele ; Fiore, Stefano ; Amato, Concetta ; Triassi, Maria. / Large-Scale Survey of Human Enteroviruses in Wastewater Treatment Plants of a Metropolitan Area of Southern Italy. In: Food and Environmental Virology. 2018 ; Vol. 10, No. 2. pp. 187-192.
@article{e0d58b98321543688a468c48ad2fde0f,
title = "Large-Scale Survey of Human Enteroviruses in Wastewater Treatment Plants of a Metropolitan Area of Southern Italy",
abstract = "Human enteroviruses (HEVs) occur in high concentrations in wastewater and can contaminate receiving environmental waters, constituting a major cause of acute waterborne disease worldwide. In this study, we investigated the relative abundance, occurrence, and seasonal distribution of polio and other enteroviruses at three wastewater treatment plants (WWTPs) in Naples, Southern Italy, from January 2010 to December 2014. Influent and effluent samples from the three WWTPs were collected monthly. One hundred and sixty-one of the 731 wastewater samples collected (22.0{\%}) before and after water treatment were CPE positive on RD cells; while no samples were positive on L20B cells from any WWTPs. Among the 140 non-polio enterovirus isolated from inlet sewage, 69.3{\%} were Coxsackieviruses type B and 30.7{\%} were Echoviruses. Among these, CVB3 and CVB5 were most prevalent, followed by CVB4 and Echo6. The twenty-one samples tested after treatment contained 6 CVB4, 5 CVB3, 3 Echo11, and 2 Echo6; while other serotypes were isolated less frequently. Data on viral detection in treated effluents of WWTPs confirmed the potential environmental contamination by HEVs and could be useful to establish standards for policies on wastewater management.",
keywords = "Enterovirus/isolation & purification, Enterovirus Infections/virology, Humans, Italy, Poliomyelitis/virology, Poliovirus/isolation & purification, Sewage/virology, Surveys and Questionnaires, Waste Water/virology, Water Purification/methods",
author = "Francesca Pennino and Antonio Nardone and Paolo Montuori and Sara Aurino and Ida Torre and Andrea Battistone and Roberto Delogu and Gabriele Buttinelli and Stefano Fiore and Concetta Amato and Maria Triassi",
year = "2018",
month = "6",
doi = "10.1007/s12560-017-9331-3",
language = "English",
volume = "10",
pages = "187--192",
journal = "Food and Environmental Virology",
issn = "1867-0334",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Large-Scale Survey of Human Enteroviruses in Wastewater Treatment Plants of a Metropolitan Area of Southern Italy

AU - Pennino, Francesca

AU - Nardone, Antonio

AU - Montuori, Paolo

AU - Aurino, Sara

AU - Torre, Ida

AU - Battistone, Andrea

AU - Delogu, Roberto

AU - Buttinelli, Gabriele

AU - Fiore, Stefano

AU - Amato, Concetta

AU - Triassi, Maria

PY - 2018/6

Y1 - 2018/6

N2 - Human enteroviruses (HEVs) occur in high concentrations in wastewater and can contaminate receiving environmental waters, constituting a major cause of acute waterborne disease worldwide. In this study, we investigated the relative abundance, occurrence, and seasonal distribution of polio and other enteroviruses at three wastewater treatment plants (WWTPs) in Naples, Southern Italy, from January 2010 to December 2014. Influent and effluent samples from the three WWTPs were collected monthly. One hundred and sixty-one of the 731 wastewater samples collected (22.0%) before and after water treatment were CPE positive on RD cells; while no samples were positive on L20B cells from any WWTPs. Among the 140 non-polio enterovirus isolated from inlet sewage, 69.3% were Coxsackieviruses type B and 30.7% were Echoviruses. Among these, CVB3 and CVB5 were most prevalent, followed by CVB4 and Echo6. The twenty-one samples tested after treatment contained 6 CVB4, 5 CVB3, 3 Echo11, and 2 Echo6; while other serotypes were isolated less frequently. Data on viral detection in treated effluents of WWTPs confirmed the potential environmental contamination by HEVs and could be useful to establish standards for policies on wastewater management.

AB - Human enteroviruses (HEVs) occur in high concentrations in wastewater and can contaminate receiving environmental waters, constituting a major cause of acute waterborne disease worldwide. In this study, we investigated the relative abundance, occurrence, and seasonal distribution of polio and other enteroviruses at three wastewater treatment plants (WWTPs) in Naples, Southern Italy, from January 2010 to December 2014. Influent and effluent samples from the three WWTPs were collected monthly. One hundred and sixty-one of the 731 wastewater samples collected (22.0%) before and after water treatment were CPE positive on RD cells; while no samples were positive on L20B cells from any WWTPs. Among the 140 non-polio enterovirus isolated from inlet sewage, 69.3% were Coxsackieviruses type B and 30.7% were Echoviruses. Among these, CVB3 and CVB5 were most prevalent, followed by CVB4 and Echo6. The twenty-one samples tested after treatment contained 6 CVB4, 5 CVB3, 3 Echo11, and 2 Echo6; while other serotypes were isolated less frequently. Data on viral detection in treated effluents of WWTPs confirmed the potential environmental contamination by HEVs and could be useful to establish standards for policies on wastewater management.

KW - Enterovirus/isolation & purification

KW - Enterovirus Infections/virology

KW - Humans

KW - Italy

KW - Poliomyelitis/virology

KW - Poliovirus/isolation & purification

KW - Sewage/virology

KW - Surveys and Questionnaires

KW - Waste Water/virology

KW - Water Purification/methods

U2 - 10.1007/s12560-017-9331-3

DO - 10.1007/s12560-017-9331-3

M3 - Article

VL - 10

SP - 187

EP - 192

JO - Food and Environmental Virology

JF - Food and Environmental Virology

SN - 1867-0334

IS - 2

ER -